Evidence map›Paper›PMID 34706646›Full record

ArticleBMC genomic data2021

RNA-Seq analysis in giant pandas reveals the differential expression of multiple genes involved in cataract formation.

Yuyan You, Chao Bai, Xuefeng Liu, Yan Lu, Ting Jia, Maohua Xia, Yanqiang Yin, Wei Wang, Yucun Chen, Chenglin Zhang and 11 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomic data, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 2 institutions in 1 country.

Yuyan You *Beijing Key Laboratory of Captive Wildlife Technologies, Beijing Zoo, Beijing, China. youyy351@163.com.
Chao Bai *Beijing Key Laboratory of Captive Wildlife Technologies, Beijing Zoo, Beijing, China.
Xuefeng LiuBeijing Zoo, Beijing, China. lxf9722@163.com.
Yan LuBeijing Key Laboratory of Captive Wildlife Technologies, Beijing Zoo, Beijing, China.
Ting JiaBeijing Zoo, Beijing, China.
Maohua XiaBeijing Zoo, Beijing, China.
Yanqiang YinChongqing Zoo, Chongqing, China.
Wei WangBeijing Key Laboratory of Captive Wildlife Technologies, Beijing Zoo, Beijing, China.
Yucun ChenStrait (Fuzhou) Giant Panda Research and Exchange Centers, Fuzhou, China.
Chenglin ZhangBeijing Key Laboratory of Captive Wildlife Technologies, Beijing Zoo, Beijing, China.
Yan LiuBeijing Key Laboratory of Captive Wildlife Technologies, Beijing Zoo, Beijing, China.
Liqin WangChengdu Zoo, Chengdu, China.
Tianchun PuBeijing Zoo, Beijing, China.
Tao MaBeijing Zoo, Beijing, China.
Yanhui LiuBeijing Zoo, Beijing, China.
Jun ZhouChongqing Zoo, Chongqing, China.
Lili NiuChengdu Zoo, Chengdu, China.
Suhui XuStrait (Fuzhou) Giant Panda Research and Exchange Centers, Fuzhou, China.
Yanxia NiBeijing Zoo, Beijing, China.
Xin HuBeijing Key Laboratory of Captive Wildlife Technologies, Beijing Zoo, Beijing, China.
Zengshuai ZhangBeijing Zoo, Beijing, China.
Guangzhou Zoo · CNChongqing Academy of Animal Science · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe giant panda (Ailuropoda melanoleuca) is an endangered mammalian species native to China. Fewer than 2500 giant pandas are known to exist, many of which are bred in captivity as a means to preserve and repopulate the species. Like other captive mammals, giant pandas acquire age-related cataracts, reducing their quality of life. Recent comparative genome-wide methylation analysis revealed 110 differentially methylated genes associated with cataract formation including six also associated with the formation of age-related cataracts in humans.

resultsTo investigate the pathological pathway in greater detail, here we used RNA-Seq analysis to investigate the differential expression profiles of genes in three giant pandas with cataracts and three healthy controls. We identified more than 700 differentially expressed genes, 29 of which were selected for further analysis based on their low q-value. We found that many of the genes encoded regulatory and signaling proteins associated with the control of cell growth, migration, differentiation and apoptosis, supporting previous research indicating a key role for apoptosis in cataract formation.

conclusionThe identification of genes involved in the formation of age-related cataracts could facilitate the development of predictive markers, preventative measures and even new therapies to improve the life of captive animals.

Indexed as

Gene ExpressionRNA-SeqAnimalsCataractEndangered SpeciesFemaleMaleQuality of LifeUrsidaeCataractsGiant panda, endangered mammalsRNA-Seq

Identifiers

PMID34706646
PMCPMC8555103
OpenAlexW3210503887

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.