Evidence mapPaperPMID 34788810Full record

ArticleHuman molecular genetics2022

Associations of carotid intima media thickness with gene expression in whole blood and genetically predicted gene expression across 48 tissues.

Andy B Castaneda, Lauren E Petty, Markus Scholz, Rick Jansen, Stefan Weiss, Xiaoling Zhang, Katharina Schramm, Frank Beutner, Holger Kirsten, Ulf Schminke and 37 more

Abstract read
In one paragraph

Article in Human molecular genetics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

47 authors.

Andy B CastanedaHuman Genetics Center, Department of Epidemiology, Human Genetics, and Environmental Sciences, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX, USA.
Lauren E PettyVanderbilt Genetics Institute, Vanderbilt University Medical Center, Nashville, TN, USA.
Markus ScholzInstitute for Medical Informatics, Statistics and Epidemiology, University of Leipzig, Leipzig, Germany.
Rick JansenDepartment of Psychiatry, VU University Medical Center, Amsterdam, the Netherlands.
Stefan WeissInterfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
Xiaoling ZhangDepartment of Medicine, Boston University School of Medicine, Boston, MA, USA.
Katharina SchrammInstitute of Neurogenomics, Helmholz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany.
Frank BeutnerHeart Center Leipzig, Leipzig, Germany.
Holger KirstenInstitute for Medical Informatics, Statistics and Epidemiology, University of Leipzig, Leipzig, Germany.
Ulf SchminkeDepartment of Neurology, University Medicine Greifswald, Greifswald, Germany.
Shih-Jen HwangThe Framingham Heart Study, Framingham, MA, USA.
Carola MarziInstitute of Epidemiology, Helmholz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany.
Klodian DhanaDepartment of Epidemiology, Erasmus University Medical Center, Rotterdam, the Netherlands.
Adrie SeldenrijkDepartment of Psychiatry, VU University Medical Center, Amsterdam, the Netherlands.
Knut KrohnInterdisciplinary Center of Clinical Research, University of Leipzig, Leipzig, Germany.
Georg HomuthInterfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald, Greifswald, Germany.
Petra WolfInstitute of Neurogenomics, Helmholz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany.
Marjolein J PetersDepartment of Internal Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands.
Marcus DörrDZHK (German Center for Cardiovascular Research), partner site Greifswald, Greifswald, Germany.
Annette PetersInstitute of Epidemiology, Helmholz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany.
Joyce B J van MeursDepartment of Internal Medicine, Erasmus University Medical Center, Rotterdam, the Netherlands.
André G UitterlindenDepartment of Epidemiology, Erasmus University Medical Center, Rotterdam, the Netherlands.
Maryam KavousiDepartment of Epidemiology, Erasmus University Medical Center, Rotterdam, the Netherlands.
Daniel LevyThe Framingham Heart Study, Framingham, MA, USA.
Christian HerderInstitute of Clinical Diabetology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Gerard van GrootheestDepartment of Psychiatry, VU University Medical Center, Amsterdam, the Netherlands.
Melanie WaldenbergerInstitute of Epidemiology, Helmholz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany.
Christa MeisingerInstitute of Epidemiology, Helmholz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany.
Wolfgang RathmannInstitute of Biometrics and Epidemiology, German Diabetes Center, Leibniz Center for Diabetes Research at Heinrich Heine University Düsseldorf, Düsseldorf, Germany.
Joachim ThieryLIFE Research Center of Civilization Diseases, University of Leipzig, Leipzig, Germany.
Joseph PolakTufts University School of Medicine, Boston, MA, USA.
Wolfgang KoenigDeutsches Herzzentrum München, Technische Universität München, Munich, Germany.
Jochen SeisslerDiabetes Center, Diabetes Research Group, Medizinische Klinik und Poliklinik IV, Ludwig-Maximilians-Universität, Munich, Germany.
Joshua C BisCardiovascular Health Research Unit, Department of Medicine, University of Washington, Seattle, WA, USA.
Nora FranceshiniDepartment of Epidemiology, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, USA.
Claudia GiambartolomeiIstituto Italiano di Tecnologia (IIT), Central RNA Lab, Genova, Italy.
Cohorts for Heart and Aging Research in Genomic Epidemiology (CHARGE) Subclinical Working Group
Albert HofmanDepartment of Epidemiology, Erasmus University Medical Center, Rotterdam, the Netherlands.
Oscar H FrancoDepartment of Epidemiology, Erasmus University Medical Center, Rotterdam, the Netherlands.
Brenda W J H PenninxDepartment of Psychiatry, VU University Medical Center, Amsterdam, the Netherlands.
Holger ProkischInstitute of Neurogenomics, Helmholz Zentrum München - German Research Center for Environmental Health, Neuherberg, Germany.
Henry VölzkeDZHK (German Center for Cardiovascular Research), partner site Greifswald, Greifswald, Germany.
Markus LoefflerInstitute for Medical Informatics, Statistics and Epidemiology, University of Leipzig, Leipzig, Germany.
Christopher J O'DonnellThe Framingham Heart Study, Framingham, MA, USA.
Jennifer E BelowVanderbilt Genetics Institute, Vanderbilt University Medical Center, Nashville, TN, USA.
Abbas DehghanDepartment of Epidemiology, Erasmus University Medical Center, Rotterdam, the Netherlands.
Paul S de VriesHuman Genetics Center, Department of Epidemiology, Human Genetics, and Environmental Sciences, School of Public Health, The University of Texas Health Science Center at Houston, Houston, TX, USA.

Funding

Framingham Project/LevyZIAHL006001 · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · 2025 to 2025
$3.9M
Hispanic Latino Lipid ConsortiumR01HL142302 · VANDERBILT UNIVERSITY MEDICAL CENTER · 2025 to 2025
$652k
The Role of Arylsulfatase in Vascular CalcificationR01HL162928 · MASSACHUSETTS GENERAL HOSPITAL · 2025 to 2025
$629k
Medical Research Council MC_PC_17228Medical Research Council MC_QA137853Medical Research Council MR/S019669/1NHLBI NIH HHS R01 HL105756NHLBI NIH HHS R01 HL142302NHLBI NIH HHS R01 HL146860NHLBI NIH HHS R01 HL162928NIA NIH HHS RF1 AG061351NIDDK NIH HHS R01 DK117445NIGMS NIH HHS R01 GM133169NIMHD NIH HHS R01 MD012765
6 · The paper itself

Abstract

Carotid intima media thickness (cIMT) is a biomarker of subclinical atherosclerosis and a predictor of future cardiovascular events. Identifying associations between gene expression levels and cIMT may provide insight to atherosclerosis etiology. Here, we use two approaches to identify associations between mRNA levels and cIMT: differential gene expression analysis in whole blood and S-PrediXcan. We used microarrays to measure genome-wide whole blood mRNA levels of 5647 European individuals from four studies. We examined the association of mRNA levels with cIMT adjusted for various potential confounders. Significant associations were tested for replication in three studies totaling 3943 participants. Next, we applied S-PrediXcan to summary statistics from a cIMT genome-wide association study (GWAS) of 71 128 individuals to estimate the association between genetically determined mRNA levels and cIMT and replicated these analyses using S-PrediXcan on an independent GWAS on cIMT that included 22 179 individuals from the UK Biobank. mRNA levels of TNFAIP3, CEBPD and METRNL were inversely associated with cIMT, but these associations were not significant in the replication analysis. S-PrediXcan identified associations between cIMT and genetically determined mRNA levels for 36 genes, of which six were significant in the replication analysis, including TLN2, which had not been previously reported for cIMT. There was weak correlation between our results using differential gene expression analysis and S-PrediXcan. Differential expression analysis and S-PrediXcan represent complementary approaches for the discovery of associations between phenotypes and gene expression. Using these approaches, we prioritize TNFAIP3, CEBPD, METRNL and TLN2 as new candidate genes whose differential expression might modulate cIMT.

Indexed as

AtherosclerosisCarotid Intima-Media ThicknessGene ExpressionGenome-Wide Association StudyHumansRisk Factors

Identifiers

PMID34788810
PMCPMC8976428

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.