ArticleNature communications2021
Self-organization of human dorsal-ventral forebrain structures by light induced SHH.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
- Synthetic developmental engineering of human liver organogenesis.Development (Cambridge, England) · 2026Review
- Anti-asthma drug montelukast induces autistic behaviors via disrupting neuronal retinoic acid signaling.Signal transduction and targeted therapy · 2026Article
- Light- and chemical-induced ciliary signaling governs dorsal/ventral regionalization of human telencephalic organoids.Nature communications · 2026Article
- Lights up on the embryonic dance: tools and applications of optogenetics in developmental biology.Genes & development · 2026Review
- Engineering human neuronal diversity: Morphogens and stem cell technologies for neurodevelopmental biology.Stem cell reports · 2025Review
- Progenitor neighborhoods function as transient niches to sustain olfactory neurogenesis.Stem cell reports · 2025Article
- Unique metabolic regulation of micromeres contributes to gastrulation in the sea urchin embryo.Nature communications · 2025Article
- Midline assembloids reveal regulators of human axon guidance.Science (New York, N.Y.) · 2025Article
- Induced Neural Progenitor Specification from Human Pluripotent Stem Cells by a Refined Synthetic Notch Platform.ACS synthetic biology · 2025Article
- Toxicity assessment using neural organoids: innovative approaches and challenges.Toxicological research · 2025Review
- Probing the molecular and cellular pathological mechanisms of schizophrenia using human induced pluripotent stem cell models.Schizophrenia research · 2024Review
- Templated Pluripotent Stem Cell Differentiation via Substratum-Guided Artificial Signaling.ACS biomaterials science & engineering · 2024Article
- Retinoic acid induces human gastruloids with posterior embryo-like structures.Nature cell biology · 2024Article
- Multiscale engineering of brain organoids for disease modeling.Advanced drug delivery reviews · 2024Review
- Correspondence between multiple signaling and developmental cellular patterns: a computational perspective.Frontiers in cell and developmental biology · 2024Article
- Spatiotemporal, optogenetic control of gene expression in organoids.Nature methods · 2023Article
- Quantitative insights in tissue growth and morphogenesis with optogenetics.Physical biology · 2023Review
- Comparison of transformations for single-cell RNA-seq data.Nature methods · 2023Article
- How might we build limbsFrontiers in cell and developmental biology · 2023Review
- Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organizing centers secrete morphogens that specify the emergence of germ layers and the establishment of the body's axes during embryogenesis. While traditional experimental embryology tools have been instrumental in dissecting the molecular aspects of organizers in model systems, they are impractical in human in-vitro model systems to dissect the relationships between signaling and fate along embryonic coordinates. To systematically study human embryonic organizer centers, we devised a collection of optogenetic ePiggyBac vectors to express a photoactivatable Cre-loxP recombinase, that allows the systematic induction of organizer structures by shining blue-light on human embryonic stem cells (hESCs). We used a light stimulus to geometrically confine SHH expression in neuralizing hESCs. This led to the self-organization of mediolateral neural patterns. scRNA-seq analysis established that these structures represent the dorsal-ventral forebrain, at the end of the first month of development. Here, we show that morphogen light-stimulation is a scalable tool that induces self-organizing centers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.