Evidence mapPaperPMID 34800086Full record

ArticleJournal of diabetes investigation2022

miR-4431 targets TRIP10/PRKD1 and impairs glucose metabolism.

Chongge Pan, Menghuan Li, Jingzhou Wang, Xiaolong Chu, Jianyu Xiong, Xin Yang, Yihan Tang, Dingling Ma, Chenggang Yuan, Jiaojiao Zhu and 3 more

Open access · goldAbstract read
In one paragraph

Article in Journal of diabetes investigation, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.

  1. Pooled it
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  3. Article
  4. Article
  5. Article
  6. Genetic factors associated with erectile dysfunction- mendelian randomisation analysis.American journal of clinical and experimental urology · 2025
    Article
  7. Review
  8. Review
  9. Review
  10. miR-4431 targets TRIP10/PRKD1 and impairs glucose metabolism.Journal of diabetes investigation · 2022
    Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 1 institution in 1 country.

Chongge PanDepartment of Biochemistry and Molecular Biology, Shihezi University School of Medicine, Shihezi, China.
Menghuan LiShihezi University School of Medicine, Shihezi, China.
Jingzhou WangDepartment of Biochemistry and Molecular Biology, Shihezi University School of Medicine, Shihezi, China.
Xiaolong ChuDepartment of Biochemistry and Molecular Biology, Shihezi University School of Medicine, Shihezi, China.
Jianyu XiongDepartment of Biochemistry and Molecular Biology, Shihezi University School of Medicine, Shihezi, China.
Xin YangDepartment of Biochemistry and Molecular Biology, Shihezi University School of Medicine, Shihezi, China.
Yihan TangDepartment of Biochemistry and Molecular Biology, Shihezi University School of Medicine, Shihezi, China.
Dingling MaDepartment of Biochemistry and Molecular Biology, Shihezi University School of Medicine, Shihezi, China.
Chenggang YuanDepartment of Biochemistry and Molecular Biology, Shihezi University School of Medicine, Shihezi, China.
Jiaojiao ZhuDepartment of Biochemistry and Molecular Biology, Shihezi University School of Medicine, Shihezi, China.
Yongsheng ChangShihezi University School of Medicine, Shihezi, China.
Jun ZhangDepartment of Biochemistry and Molecular Biology, Shihezi University School of Medicine, Shihezi, China.ORCID https://orcid.org/0000-0001-9796-994X
Cuizhe WangMinistry of Education Key Laboratory of Xinjiang Endemic and Ethnic Disease, Shihezi, China.ORCID https://orcid.org/0000-0002-3338-9732
Shihezi University · CN

Funding

National Natural Science Foundation of China 81900707National Natural Science Foundation of China 81960152National Natural Science Foundation of China 82160156Projects of Shihezi University GJHZ201703Scientific and Technological Research Project of Xinjiang Production and Construction Corps 2018AB018Scientific and Technological Research Project of Xinjiang Production and Construction Corps 2021AB028Xinjiang Production and Construction Corps Key Areas Innovation Team Project 2018CB002
6 · The paper itself

Abstract

AIM/

introductionObesity is considered an important risk factor for many metabolic disorders, especially type 2 diabetes mellitus, and microRNAs (miRNAs) play a vital role in the development of type 2 diabetes mellitus. Therefore, we conducted this study to investigate the role of miR-4431 in the obesity-associated pathobiology of type 2 diabetes mellitus. MATERIALS AND

methodsSubjects were divided into normal control (n = 36), obese (n = 36), and type 2 diabetes mellitus (n = 12) groups, and serum miR-4431 levels were analyzed. Adenovirus-vectored miR-4431 mimic or sponge was intraperitoneally injected into the normal diet group and the high-fat diet group (HFD) mice to investigate glucose tolerance, insulin sensitivity, and lipid levels. The downstream target genes of miR-4431 were predicted using bioinformatics, and they were verified in vitro.

resultsSerum miR-4431 levels were significantly high in obese and type 2 diabetes mellitus individuals, and positively correlated with the body mass index and fasting plasma glucose levels. In HFD mice, miR-4431 levels in the serum, white adipose tissue, and liver were significantly increased. Moreover, miR-4431 impaired glucose tolerance, insulin sensitivity, and lipid metabolism in mice. Bioinformatic prediction suggested that TRIP10 and PRKD1 could be the downstream target genes of miR-4431. The HFD mice showed a remarkable reduction in the mRNA levels of TRIP10 and PRKD1 in the liver, which were countered by blocking miR-4431. In HepG2 and L02 cells, miR-4431 could downregulate TRIP10 and PRKD1 while blocking glucose uptake. The luciferase reporter assay showed that miR-4431 could bind TRIP10 and PRKD1 3'-UTR.

conclusionmiR-4431 targets TRIP10/PRKD1 and impairs glucose metabolism.

Indexed as

Diabetes Mellitus, Type 2Insulin ResistanceMicroRNAsAnimalsDiet, High-FatGlucoseHumansLipid MetabolismMiceMice, Inbred C57BLObesityGlucoseMicroRNAsGlucose metabolismmiR-4431Type 2 diabetes mellitus

Identifiers

PMID34800086
PMCPMC9017615
OpenAlexW3214148084

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.