Evidence mapPaperPMID 34823555Full record

ArticleLipids in health and disease2021

Accumulation of LDL/ox-LDL in the necrotic region participates in osteonecrosis of the femoral head: a pathological and in vitro study.

Xin-Yuan Wang, Tian-Le Ma, Kang-Ning Chen, Zhi-Ying Pang, Hao Wang, Jun-Ming Huang, Guo-Bin Qi, Chen-Zhong Wang, Zeng-Xin Jiang, Lin-Jing Gong and 3 more

Open access · goldAbstract read
In one paragraph

Article in Lipids in health and disease, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Clinical Characteristics, Current Treatment Options, Potential Mechanisms, Biomarkers, and Therapeutic Targets in Avascular Necrosis of Femoral Head.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Xin-Yuan Wang *Department of Orthopaedics, West China Hospital, West China Medical School, Sichuan University, Chengdu, 610041, Sichuan Province, China.
Tian-Le Ma *Department of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Kang-Ning Chen *Department of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Zhi-Ying PangDepartment of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Hao WangDepartment of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Jun-Ming HuangDepartment of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Guo-Bin QiDepartment of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Chen-Zhong WangDepartment of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Zeng-Xin JiangDepartment of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Lin-Jing GongDepartment of Respiratory and Critical Care Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.
Zhe WangDepartment of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China.
Chang JiangDepartment of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China. cjiang_fdu@yeah.net.
Zuo-Qin YanDepartment of Orthopaedics, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China. yan.zuoqin@zs-hospital.sh.cn.ORCID http://orcid.org/0000-0001-8704-9728
Fudan University · CNSichuan University · CN

Funding

National Natural Science Foundation of China 81871742National Natural Science Foundation of China 82172413Shanghai Hospital Development Center Emerging Advanced Technology Joint Research Project SHDC12017107Shanghai Hospital Development Center Three-year Action Plan Major Clinical Research Project SHDC2020CR3075B
6 · The paper itself

Abstract

backgroundOsteonecrosis of the femoral head (ONFH) is a common but intractable disease that appears to involve lipid metabolic disorders. Although numerous studies have demonstrated that high blood levels of low-density lipoprotein (LDL) are closely associated with ONFH, there is limited evidence to explain the pathological role of LDL. Pathological and in vitro studies were performed to investigate the role of disordered metabolism of LDL and oxidized LDL (ox-LDL) in the femoral head in the pathology of ONFH.

methodsNineteen femoral head specimens from patients with ONFH were obtained for immunohistochemistry analysis. Murine long-bone osteocyte Y4 cells were used to study the effects of LDL/ox-LDL on cell viability, apoptosis, and metabolism process of LDL/ox-LDL in osteocytes in normoxic and hypoxic environments.

resultsIn the pathological specimens, marked accumulation of LDL/ox-LDL was observed in osteocytes/lacunae of necrotic regions compared with healthy regions. In vitro studies showed that ox-LDL, rather than LDL, reduced the viability and enhanced apoptosis of osteocytes. Pathological sections indicated that the accumulation of ox-LDL was significantly associated with impaired blood supply. Exposure to a hypoxic environment appeared to be a key factor leading to LDL/ox-LDL accumulation by enhancing internalisation and oxidation of LDL in osteocytes.

conclusionsThe accumulation of LDL/ox-LDL in the necrotic region may contribute to the pathology of ONFH. These findings could provide new insights into the prevention and treatment of ONFH.

Indexed as

Femur Head NecrosisFluorescent Antibody TechniqueHumansLipoproteins, LDLOsteocytesReal-Time Polymerase Chain ReactionLipoproteins, LDLoxidized low density lipoproteinHypoxiaLow-density lipoproteinOsteocyteOsteonecrosis of the femoral headOxidized low-density lipoprotein

Identifiers

PMID34823555
PMCPMC8620162
OpenAlexW3216751155

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.