Evidence map›Paper›PMID 34830008›Full record

ArticleInternational journal of molecular sciences2021

Global Transcriptomic Analysis Reveals Differentially Expressed Genes Involved in Embryogenic Callus Induction in Drumstick (

Endian Yang, Mingyang Zheng, Xuan Zou, Xiaoling Huang, Heyue Yang, Xiaoyang Chen, Junjie Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.3field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Genome-Wide Identification and Expression Analysis of theInternational journal of molecular sciences · 2022
    Article
  5. Miracle TreePlants (Basel, Switzerland) · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Endian YangDepartment of Forestry, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Mingyang ZhengDepartment of Forestry, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Xuan ZouDepartment of Forestry, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Xiaoling HuangDepartment of Forestry, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Heyue YangDepartment of Forestry, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Xiaoyang ChenDepartment of Forestry, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
Junjie ZhangDepartment of Forestry, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China.
South China Agricultural University · CNKey Laboratory of Guangdong Province · CN

Funding

Department of Education of Guangdong Province 2019KQNCX010Department of Forestry of Guangdong Province 2018KJCX001
6 · The paper itself

Abstract

The plant embryogenic callus (EC) is an irregular embryogenic cell mass with strong regenerative ability that can be used for propagation and genetic transformation. However, difficulties with EC induction have hindered the breeding of drumstick, a tree with diverse potential commercial uses. In this study, three drumstick EC cDNA libraries were sequenced using an Illumina NovaSeq 6000 system. A total of 7191 differentially expressed genes (DEGs) for embryogenic callus development were identified, of which 2325 were mapped to the KEGG database, with the categories of plant hormone signal transduction and Plant-pathogen interaction being well-represented. The results obtained suggest that auxin and cytokinin metabolism and several embryogenesis-labeled genes are involved in embryogenic callus induction. Additionally, 589 transcription factors from 20 different families were differentially expressed during EC formation. The differential expression of 16 unigenes related to auxin signaling pathways was validated experimentally by quantitative real time PCR (qRT-PCR) using samples representing three sequential developmental stages of drumstick EC, supporting their apparent involvement in drumstick EC formation. Our study provides valuable information about the molecular mechanism of EC formation and has revealed new genes involved in this process.

Indexed as

Bony CallusGene Expression ProfilingGene Expression Regulation, PlantIndoleacetic AcidsMoringa oleiferaPlant Growth RegulatorsPlant ProteinsPlant Somatic Embryogenesis TechniquesTranscriptomeIndoleacetic AcidsPlant Growth RegulatorsPlant Proteinsdifferentially expressed genesembryogenic callusMoringa oleiferatranscriptome

Identifiers

PMID34830008
PMCPMC8619801
OpenAlexW3212350654

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.