ArticleNature reviews. Methods primers2021
Optical tweezers in single-molecule biophysics.
Article in Nature reviews. Methods primers, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 209 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
209 citing papers in PubMed.
- A review of the wide applications of microfluidic devices in peri-implantitis, periodontitis, and endodontics.Odontology · 2026Review
- Concurrent positional dynamics and activity mapping of DNA-binding proteins.Nature protocols · 2026Review
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- Multivalent cations stabilize DNA duplexes beyond charge neutralization.Chemical science · 2026Article
- Denoise Stepwise Signals by Diffusion Model-Based Approach.Small methods · 2026Article
- Cellular Responses to Mechanical Cues Across Scales: From Fundamental Insights to Translational Potential.Advanced healthcare materials · 2026Review
- Lipid Bilayer-Confined J-Aggregation Transduces Cell Membrane Mechanics into Photoacoustic Signals.Journal of the American Chemical Society · 2026Article
- Force Reveals Hidden Conformations and Dissociation Pathways in Individual π-Interacting Dimers.Angewandte Chemie (International ed. in English) · 2026Article
- Current Challenges of Transcription Compartmentalization Research.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Drop-Shaped Optical Microfiber Enabled Biomechanical Sensor.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Twist-encoded magnetic plasmon interferometry for label-free, orientation-resolved single-molecule imaging.Science advances · 2026Article
- Label-Free Single Protein Dynamics Revealed by Metasurface-Enhanced Raman Spectroscopy.ACS nano · 2026Article
- Structural and Thermodynamic Properties of RNA Molecules Using a Knowledge-Based Model.Journal of chemical theory and computation · 2026Article
- Dpb11 facilitates the colocalization of Mec1-Ddc2 with its activators on gapped DNA.Cell reports · 2026Article
- Red Blood Cells as Endogenous Biotweezers for Optical Micromanipulation In Vivo.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Programmable rotation of single cells along arbitrary axes via opto-thermo-osmotic torque.Light, science & applications · 2026Article
- Plasmonic Optical Tweezers and Surface-Enhanced Raman Spectroscopy: Fundamentals, Single-Entity Applications, and the Evolving Role of Artificial Intelligence.Bioengineering (Basel, Switzerland) · 2026Review
- Review
- A Nanopore-Gated Subattoliter Silicon Nanocavity for Single-Molecule Trapping and Analysis without Applying an External Force.ACS nano · 2026Article
- SmartTrap: automated precision experiments with optical tweezers.Nature methods · 2026Article
149 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Optical tweezers have become the method of choice in single-molecule manipulation studies. In this Primer, we first review the physical principles of optical tweezers and the characteristics that make them a powerful tool to investigate single molecules. We then introduce the modifications of the method to extend the measurement of forces and displacements to torques and angles, and to develop optical tweezers with single-molecule fluorescence detection capabilities. We discuss force and torque calibration of these instruments, their various modes of operation and most common experimental geometries. We describe the type of data obtained in each experimental design and their analyses. This description is followed by a survey of applications of these methods to the studies of protein-nucleic acid interactions, protein/RNA folding and molecular motors. We also discuss data reproducibility, the factors that lead to the data variability among different laboratories and the need to develop field standards. We cover the current limitations of the methods and possible ways to optimize instrument operation, data extraction and analysis, before suggesting likely areas of future growth.
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.