ArticleLab on a chip2021
A multi-functional microfluidic device compatible with widefield and light sheet microscopy.
Article in Lab on a chip, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.Nano letters · 2026Article
- Adapting upright light sheet fluorescence microscopy for imaging at air-liquid interface.Npj imaging · 2026Article
- A multimodal adaptive optical microscope for in vivo imaging from molecules to organisms.Nature methods · 2026Article
- Polarization-engineered aberration-resilient light sheet microscopy.bioRxiv : the preprint server for biology · 2026Article
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.bioRxiv : the preprint server for biology · 2026Article
- Adapting Upright Light Sheet Fluorescence Microscopy for Imaging at Air-Liquid Interface.bioRxiv : the preprint server for biology · 2026Article
- Article
- Single-Objective Lattice Light Sheet Microscopy with Microfluidics for Single-Molecule Super-Resolution Imaging of Mammalian Cells.ACS photonics · 2026Article
- Altair-LSFM A High-Resolution, Easy-to-Build Light-Sheet Microscope for Sub-Cellular Imaging.bioRxiv : the preprint server for biology · 2025Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.Nature communications · 2024Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.bioRxiv : the preprint server for biology · 2024Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- A vascularized breast cancer spheroid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy.Nature communications · 2024Article
- Smart lattice light-sheet microscopy for imaging rare and complex cellular events.Nature methods · 2024Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
We present a microfluidic device compatible with high resolution light sheet and super-resolution microscopy. The device is a 150 μm thick chamber with a transparent fluorinated ethylene propylene (FEP) cover that has a similar refractive index (1.34) to water (1.33), making it compatible with top-down imaging used in light sheet microscopy. We provide a detailed fabrication protocol and characterize the optical performance of the device. We demonstrate that the device supports long-term imaging of cell growth and differentiation as well as the rapid addition and removal of reagents while simultaneously maintaining sterile culture conditions by physically isolating the sample from the dipping lenses used for imaging. Finally, we demonstrate that the device can be used for super-resolution imaging using lattice light sheet structured illumination microscopy (LLS-SIM) and DNA PAINT. We anticipate that FEP-based microfluidics, as shown here, will be broadly useful to researchers using light sheet microscopy due to the ability to switch reagents, image weakly adherent cells, maintain sterility, and physically isolate the specimen from the optics of the instruments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.