Evidence mapPaperPMID 34873191Full record

ArticleScientific reports2021

reString: an open-source Python software to perform automatic functional enrichment retrieval, results aggregation and data visualization.

Stefano Manzini, Marco Busnelli, Alice Colombo, Elsa Franchi, Pasquale Grossano, Giulia Chiesa

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Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Stefano Manzini *Department of Pharmacological and Biomolecular Sciences, Università Degli Studi Di Milano, Milan, Italy. stefano.manzini@unimi.it.
Marco Busnelli *Department of Pharmacological and Biomolecular Sciences, Università Degli Studi Di Milano, Milan, Italy.
Alice ColomboDepartment of Pharmacological and Biomolecular Sciences, Università Degli Studi Di Milano, Milan, Italy.
Elsa FranchiDepartment of Pharmacological and Biomolecular Sciences, Università Degli Studi Di Milano, Milan, Italy.
Pasquale GrossanoFondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, Italy.
Giulia ChiesaDepartment of Pharmacological and Biomolecular Sciences, Università Degli Studi Di Milano, Milan, Italy. giulia.chiesa@unimi.it.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Functional enrichment analysis is an analytical method to extract biological insights from gene expression data, popularized by the ever-growing application of high-throughput techniques. Typically, expression profiles are generated for hundreds to thousands of genes/proteins from samples belonging to two experimental groups, and after ad-hoc statistical tests, researchers are left with lists of statistically significant entities, possibly lacking any unifying biological theme. Functional enrichment tackles the problem of putting overall gene expression changes into a broader biological context, based on pre-existing knowledge bases of reference: database collections of known expression regulation, relationships and molecular interactions. STRING is among the most popular tools, providing both protein-protein interaction networks and functional enrichment analysis for any given set of identifiers. For complex experimental designs, manually retrieving, interpreting, analyzing and abridging functional enrichment results is a daunting task, usually performed by hand by the average wet-biology researcher. We have developed reString, a cross-platform software that seamlessly retrieves from STRING functional enrichments from multiple user-supplied gene sets, with just a few clicks, without any need for specific bioinformatics skills. Further, it aggregates all findings into human-readable table summaries, with built-in features to easily produce user-customizable publication-grade clustermaps and bubble plots. Herein, we outline a complete reString protocol, showcasing its features on a real use-case.

Indexed as

Cluster AnalysisGene Expression RegulationPattern Recognition, AutomatedAnimalsAortaComputational BiologyDatabases, GeneticData MiningGene Expression ProfilingHumansInternetMicePolymerase Chain ReactionProgramming LanguagesProtein Interaction MapsProteinsProteins

Identifiers

PMID34873191
PMCPMC8648753

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.