Evidence map›Paper›PMID 34876240›Full record

ArticleHereditas2021

TGF-β1/SMOC2/AKT and ERK axis regulates proliferation, migration, and fibroblast to myofibroblast transformation in lung fibroblast, contributing with the asthma progression.

Yuebin Wang, Huike Yang, Xian Su, Anqiang Cao, Feng Chen, Peng Chen, Fangtao Yan, Huirong Hu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Hereditas, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.4field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 25 citations in OpenAlex.

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  12. Matricellular proteins in cutaneous wound healing.Frontiers in cell and developmental biology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Yuebin WangDepartment of Respiratory and Critical Care Medicine, Chengdu Third People's Hospital, Chengdu City, 610031, Sichuan Province, China.
Huike YangDepartment of Anatomy, Harbin Medical University, Harbin City, 150081, Heilongjiang Province, China.
Xian SuDepartment of Respiratory and Critical Care Medicine, Chengdu Third People's Hospital, Chengdu City, 610031, Sichuan Province, China.
Anqiang CaoDepartment of Cardiothoracic Surgery, Chengdu Third People's Hospital, No.82, Qinglong Street, Qingyang District, Chengdu City, Sichuan Province, China. caoanqiang_dr@163.com.ORCID http://orcid.org/0000-0002-5679-2460
Feng ChenDepartment of Cardiothoracic Surgery, Chengdu Third People's Hospital, No.82, Qinglong Street, Qingyang District, Chengdu City, Sichuan Province, China.
Peng ChenDepartment of Cardiothoracic Surgery, Chengdu Third People's Hospital, No.82, Qinglong Street, Qingyang District, Chengdu City, Sichuan Province, China.
Fangtao YanDepartment of Cardiothoracic Surgery, Chengdu Third People's Hospital, No.82, Qinglong Street, Qingyang District, Chengdu City, Sichuan Province, China.
Huirong HuDepartment of Cardiothoracic Surgery, Chengdu Third People's Hospital, No.82, Qinglong Street, Qingyang District, Chengdu City, Sichuan Province, China.
Third People's Hospital of Chengdu · CNHarbin Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAsthma is a common chronic respiratory disease that influences 300 million people all over the world. However, the pathogenesis of asthma has not been fully elucidated. It has been reported that transforming growth factor-β (TGF-β) can activate myofibroblasts. Moreover, the fibroblast to myofibroblast transformation (FMT) can be triggered by TGF-β, which is a major mediator of subepithelial fibrosis. Secreted modular calcium-binding protein 2 (SMOC2) is a member of cysteine (SPARC) family and is involved in the progression of multiple diseases. However, its role in asthma remains poorly understood. RT-qPCR evaluated the expression of SMOC2. Bromodeoxyuridine assay and wound-healing assay detected the proliferation and migration of lung fibroblasts, respectively. IF staining was performed to assess the expression of α-smooth muscle actin (α-SMA). Western blot analysis detected the levels of proteins. Flow cytometry was utilized for determination of the number of myofibroblasts.

resultsWe found the expression of SMOC2 was upregulated by the treatment of TGF-β1 in lung fibroblasts. In addition, SMOC2 promoted the proliferation and migration of lung fibroblasts. More importantly, SMOC2 accelerated FMT of lung fibroblasts. Furthermore, SMOC2 was verified to control the activation of AKT and ERK. Rescue assays showed that the inhibition of AKT and ERK pathway reversed the promoting effect of SMOC2 overexpression on proliferation, migration and FMT in lung fibroblasts.

conclusionsThis work demonstrated that SMOC2 modulated TGF-β1-induced proliferation, migration and FMT in lung fibroblasts and may promote asthma, which potentially provided a novel therapeutic target for the management of asthma.

Indexed as

AsthmaMyofibroblastsCalcium-Binding ProteinsCell ProliferationCells, CulturedFibroblastsHumansLungProto-Oncogene Proteins c-aktTransforming Growth Factor beta1Calcium-Binding ProteinsProto-Oncogene Proteins c-aktSMOC2 protein, humanTransforming Growth Factor beta1AsthmaLung fibroblastsMyofibroblast transformationSMOC2TGF-β1

Identifiers

PMID34876240
PMCPMC8653533
OpenAlexW4200576437

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.