Evidence map›Paper›PMID 34885151›Full record

ArticleCancers2021

Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer.

Robert Wiebringhaus, Matteo Pecoraro, Heidi A Neubauer, Karolína Trachtová, Bettina Trimmel, Maritta Wieselberg, Jan Pencik, Gerda Egger, Christoph Krall, Richard Moriggl and 3 more

Open access · goldAbstract read
In one paragraph

Article in Cancers, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 14 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 4 countries.

Robert WiebringhausDepartment of Pathology, Medical University of Vienna, 1090 Vienna, Austria.ORCID 0000-0002-9959-136X
Matteo PecoraroInstitute for Research in Biomedicine, Università della Svizzera Italiana, 6500 Bellinzona, Switzerland.
Heidi A NeubauerInstitute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.ORCID 0000-0001-7372-7786
Karolína TrachtováCentral European Institute of Technology, Masaryk University, 60177 Brno, Czech Republic.
Bettina TrimmelDepartment of Pathology, Medical University of Vienna, 1090 Vienna, Austria.
Maritta WieselbergDepartment of Pathology, Medical University of Vienna, 1090 Vienna, Austria.
Jan PencikDepartment of Pathology, Medical University of Vienna, 1090 Vienna, Austria.
Gerda EggerDepartment of Pathology, Medical University of Vienna, 1090 Vienna, Austria.ORCID 0000-0003-2489-155X
Christoph KrallInstitute for Statistics, Medical University of Vienna, 1090 Vienna, Austria.
Richard MorigglInstitute of Animal Breeding and Genetics, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.
Matthias MannDepartment of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany.
Brigitte HantuschDepartment of Pathology, Medical University of Vienna, 1090 Vienna, Austria.
Lukas KennerDepartment of Pathology, Medical University of Vienna, 1090 Vienna, Austria.ORCID 0000-0003-2184-1338
Medical University of Vienna · ATUniversity of Veterinary Medicine Vienna · ATCentral European Institute of Technology · CZLMU Klinikum · DELudwig Boltzmann Institute Applied Diagnostics · ATMax Planck Institute of Biochemistry · DEUniversità della Svizzera italiana · CH

Funding

Austrian Science Fund FWF P 26011COMET Competence Center CBmed - Center for Biomarker 326 Research in Medicine FA791A0906.FFGFWF Austrian Science Fund P26011
6 · The paper itself

Abstract

We aimed to identify novel markers for aggressive prostate cancer in a STAT3-low proteomics-derived dataset of mitochondrial proteins by immunohistochemical analysis and correlation with transcriptomic data and biochemical recurrence in a STAT3 independent PCa cohort. Formalin-fixed paraffin-embedded tissue (FFPE) sample selection for proteomic analysis and tissue-microarray (TMA) generation was conducted from a cohort of PCa patients. Retrospective data analysis was performed with the same cohort. 153 proteins differentially expressed between STAT3-low and STAT3-high samples were identified. Out of these, 46 proteins were associated with mitochondrial processes including oxidative phosphorylation (OXPHOS), and 45 proteins were upregulated, including NDUFS1/ATP5O. In a STAT3 independent PCa cohort, high expression of NDUFS1/ATP5O was confirmed by immunocytochemistry (IHC) and was significantly associated with earlier biochemical recurrence (BCR). mRNA expression levels for these two genes were significantly higher in intra-epithelial neoplasia and in PCa compared to benign prostate glands. NDUFS1/ATP5O levels are increased both at the mRNA and protein level in aggressive PCa. Our results provide evidence that NDUFS1/ATP5O could be used to identify high-risk PCa patients.

Indexed as

ATP5OFFPE-proteomicsNDUFS1OXPHOSprostate cancerSTAT3transcriptomics

Identifiers

PMID34885151
PMCPMC8656993
OpenAlexW3216889086

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.