Evidence mapPaperPMID 34890278Full record

ArticleAmerican journal of physiology. Heart and circulatory physiology2022

Differential effects of obesity on visceral versus subcutaneous adipose arteries: role of shear-activated Kir2.1 and alterations to the glycocalyx.

Sang Joon Ahn, Elizabeth Le Master, James C Lee, Shane A Phillips, Irena Levitan, Ibra S Fancher

Open access · greenAbstract read
In one paragraph

Article in American journal of physiology. Heart and circulatory physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Integrating molecular and cellular components of endothelial shear stress mechanotransduction.American journal of physiology. Heart and circulatory physiology · 2024
    Review
  6. Visceral adipose of obese mice inhibits endothelial inwardly rectifying KAmerican journal of physiology. Cell physiology · 2024
    Article
  7. Electro-metabolic signaling.The Journal of general physiology · 2024
    Article
  8. Review
  9. The damaging duo: Obesity and excess dietary salt contribute to hypertension and cardiovascular disease.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2023
    Review
  10. Review
  11. New developments in translational microcirculatory research.American journal of physiology. Heart and circulatory physiology · 2022
    Review
  12. Article
  13. Guidelines on models of diabetic heart disease.American journal of physiology. Heart and circulatory physiology · 2022
    Review
  14. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Sang Joon AhnDivision of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, Chicago, Illinois.ORCID 0000-0002-2883-6980
Elizabeth Le MasterDivision of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, Chicago, Illinois.
James C LeeDepartment of Biomedical Engineering, University of Illinois at Chicago, Chicago, Illinois.
Shane A PhillipsDepartment of Physical Therapy, College of Applied Health Sciences, University of Illinois at Chicago, Chicago, Illinois.
Irena LevitanDivision of Pulmonary, Critical Care, Sleep and Allergy, Department of Medicine, University of Illinois at Chicago, Chicago, Illinois.
Ibra S FancherDepartment of Kinesiology and Applied Physiology, College of Health Sciences, University of Delaware, Newark, Delaware.ORCID 0000-0003-4677-6755
University of Illinois Chicago · USUniversity of Delaware · US

Funding

Delaware INBRE DRPP CoreP20GM103446 · UNIVERSITY OF DELAWARE · 2025 to 2025
$4.7M
TRAINING PROGRAM IN LUNG BIOLOGY AND PATHOBIOLOGYT32HL007829 · UNIVERSITY OF ILLINOIS AT CHICAGO · 1995 to 2025
$3.4M
Cholesterol regulation of endothelial K+ channelsR01HL073965 · UNIVERSITY OF PENNSYLVANIA · 2004 to 2005
$684k
NHLBI NIH HHS R01 HL073965NHLBI NIH HHS R01 HL141120NHLBI NIH HHS T32 HL007829NHLBI NIH HHS T32 HL144909NIA NIH HHS R01 AG044404NIGMS NIH HHS P20 GM103446
6 · The paper itself

Abstract

Obesity imposes well-established deficits to endothelial function. We recently showed that obesity-induced endothelial dysfunction was mediated by disruption of the glycocalyx and a loss of Kir channel flow sensitivity. However, obesity-induced endothelial dysfunction is not observed in all vascular beds: visceral adipose arteries (VAAs), but not subcutaneous adipose arteries (SAAs), exhibit endothelial dysfunction. To determine whether differences in SAA versus VAA endothelial function observed in obesity are attributed to differential impairment of Kir channels and alterations to the glycocalyx, mice were fed a normal rodent diet, or a high-fat Western diet to induce obesity. Flow-induced vasodilation (FIV) was measured ex vivo. Functional downregulation of endothelial Kir2.1 was accomplished by transducing adipose arteries from mice and obese humans with adenovirus containing a dominant-negative Kir2.1 construct. Kir function was tested in freshly isolated endothelial cells seeded in a flow chamber for electrophysiological recordings under fluid shear. Atomic force microscopy was used to assess biophysical properties of the glycocalyx. Endothelial dysfunction was observed in VAAs of obese mice and humans. Downregulating Kir2.1 blunted FIV in SAAs, but had no effect on VAAs, from obese mice and humans. Obesity abolished Kir shear sensitivity in VAA endothelial cells and significantly altered the VAA glycocalyx. In contrast, Kir shear sensitivity was observed in SAA endothelial cells from obese mice and effects on SAA glycocalyx were less pronounced. We reveal distinct differences in Kir function and alterations to the glycocalyx that we propose contribute to the dichotomy in SAA versus VAA endothelial function with obesity.

Indexed as

AdultAnimalsArteriesCells, CulturedEndothelium, VascularGlycocalyxHumansIntra-Abdominal FatMaleMiceMice, Inbred C57BLMiddle AgedObesityPotassium Channels, Inwardly RectifyingSubcutaneous FatKir2.1 channelPotassium Channels, Inwardly Rectifyingadiposeendothelial dysfunctionglycocalyxKir2.1obesity

Identifiers

PMID34890278
PMCPMC8742723
OpenAlexW4200127495

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.