ArticleNucleic acids research2022
A simple and general approach to generate photoactivatable DNA processing enzymes.
Article in Nucleic acids research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Remote-Controlled Release of Nitric Oxide by Plasmonic Waveguide Coated With Metal-Organic Frameworks.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Light-Activated Nucleic Acid Amplification Systems Using Photo-Caged DNA Polymerase or Primers.Advanced genetics (Hoboken, N.J.) · 2026Article
- Enzyme-Integrated Hydrogels for Advanced Biological Applications.Polymer science & technology (Washington, D.C.) · 2026Review
- Universal Regulation of DNA Polymerase via Photocaged Primer Enables Light-Start Isothermal Amplification on Demand.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Nanocellulose Composites as Smart Devices With Chassis, Light-Directed DNA Storage, Engineered Electronic Properties, and Chip Integration.Frontiers in bioengineering and biotechnology · 2022Article
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Authors and funding
3 authors.
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Abstract
DNA processing enzymes, such as DNA polymerases and endonucleases, have found many applications in biotechnology, molecular diagnostics, and synthetic biology, among others. The development of enzymes with controllable activity, such as hot-start or light-activatable versions, has boosted their applications and improved the sensitivity and specificity of the existing ones. However, current approaches to produce controllable enzymes are experimentally demanding to develop and case-specific. Here, we introduce a simple and general method to design light-start DNA processing enzymes. In order to prove its versatility, we applied our method to three DNA polymerases commonly used in biotechnology, including the Phi29 (mesophilic), Taq, and Pfu polymerases, and one restriction enzyme. Light-start enzymes showed suppressed polymerase, exonuclease, and endonuclease activity until they were re-activated by an UV pulse. Finally, we applied our enzymes to common molecular biology assays and showed comparable performance to commercial hot-start enzymes.
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