Evidence map›Paper›PMID 34953002›Full record

ArticleCell proliferation2022

LncRNA-AK137033 inhibits the osteogenic potential of adipose-derived stem cells in diabetic osteoporosis by regulating Wnt signaling pathway via DNA methylation.

Shuanglin Peng, Yujin Gao, Sirong Shi, Dan Zhao, Huayue Cao, Ting Fu, Xiaoxiao Cai, Jingang Xiao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell proliferation, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.

  1. A novel regulator in cancer initiation and progression: long noncoding RNA SHNG9.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2023
    Pooled it
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  18. Biological aspects in controlling angiogenesis: current progress.Cellular and molecular life sciences : CMLS · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Shuanglin PengDepartment of Oral Implantology, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, China.
Yujin GaoDepartment of Oral Implantology, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, China.
Sirong ShiState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Dan ZhaoState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Huayue CaoDepartment of Oral Implantology, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, China.
Ting FuDepartment of Oral Implantology, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, China.
Xiaoxiao CaiState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.ORCID https://orcid.org/0000-0002-5654-7414
Jingang XiaoDepartment of Oral Implantology, The Affiliated Stomatology Hospital of Southwest Medical University, Luzhou, China.ORCID https://orcid.org/0000-0003-1300-7191
Sichuan University · CNSouthwest Medical University · CN

Funding

Joint project of Luzhou Municipal People's Government and Southwest Medical University 2020LZXNYDZ09National Natural Science Foundation of China 81870746National Natural Science Foundation of China 81970986Open Project of the State Key Laboratory of Oral Disease Research SKLOD2021OF08Project of Science & Technology Department of Sichuan Province 2018JY0399Youth Science Climbing Program of The Affiliated Stomatology Hospital of Southwest Medical University 2021KQ01
6 · The paper itself

Abstract

objectivesBone tissue engineering based on adipose-derived stem cells (ASCs) is expected to become a new treatment for diabetic osteoporosis (DOP) patients with bone defects. However, compared with control ASCs (CON-ASCs), osteogenic potential of DOP-ASCs is decreased, which increased the difficulty of bone reconstruction in DOP patients. Moreover, the cause of the poor osteogenesis of ASCs in a hyperglycemic microenvironment has not been elucidated. Therefore, this study explored the molecular mechanism of the decline in the osteogenic potential of DOP-ASCs from the perspective of epigenetics to provide a possible therapeutic target for bone repair in DOP patients with bone defects. MATERIALS AND

methodsAn animal model of DOP was established in mice. CON-ASCs and DOP-ASCs were isolated from CON and DOP mice, respectively. AK137033 small interfering RNA (SiRNA) and an AK137033 overexpression plasmid were used to regulate the expression of AK137033 in CON-ASCs and DOP-ASCs in vitro. Lentiviruses that carried shRNA-AK137033 or AK137033 cDNA were used to knockdown or overexpress AK137033, respectively, in CON-ASCs and DOP-ASCs in vivo. Hematoxylin and eosin (H&E), Masson's, alizarin red, and alkaline phosphatase (ALP) staining, micro-computed tomography (Micro-CT), flow cytometry, qPCR, western blotting, immunofluorescence, and bisulfite-specific PCR (BSP) were used to analyze the functional changes of ASCs.

resultsThe DOP mouse model was established successfully. Compared with CON-ASCs, AK137033 expression, the DNA methylation level of the sFrp2 promoter region, Wnt signaling pathway markers, and the osteogenic differentiation potential were decreased in DOP-ASCs. In vitro experiments showed that AK137033 silencing inhibited the Wnt signaling pathway and osteogenic ability of CON-ASCs by reducing the DNA methylation level in the sFrp2 promoter region. Additionally, overexpression of AK137033 in DOP-ASCs rescued these changes caused by DOP. Moreover, the same results were obtained in vivo.

conclusionsLncRNA-AK137033 inhibits the osteogenic potential of DOP-ASCs by regulating the Wnt signaling pathway via modulating the DNA methylation level in the sFrp2 promoter region. This study provides an important reference to find new targets for the treatment of bone defects in DOP patients.

Indexed as

Wnt Signaling PathwayAdipose TissueAnimalsCell DifferentiationDiabetes MellitusDisease Models, AnimalDNA MethylationGene Expression ProfilingGene Expression RegulationGene SilencingHydroxyapatitesMaleMembrane ProteinsMiceMice, Inbred C57BLOsteogenesishydroxyapatite-beta tricalcium phosphateHydroxyapatitesMembrane ProteinsRNA, Long NoncodingRNA, MessengerSecreted Frizzled-Related ProteinsSfrp2 protein, mouseadipose-derived stem cellsAK137033diabetic osteoporosisDNA methylationosteogenic potentialWnt signaling pathway

Identifiers

PMID34953002
PMCPMC8780896
OpenAlexW4200046264

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.