Evidence map›Paper›PMID 34964522›Full record

ArticleAdvanced materials (Deerfield Beach, Fla.)2022

Rapid Identification of DNA Fragments through Direct Sequencing with Electro-Optical Zero-Mode Waveguides.

Fatemeh Farhangdoust, Feng Cheng, Wentao Liang, Yongmin Liu, Meni Wanunu

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced materials (Deerfield Beach, Fla.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Fatemeh FarhangdoustDepartment of Bioengineering, Northeastern University, Boston, MA, 02115, USA.
Feng ChengDepartment of Electrical and Computer Engineering, Northeastern University, Boston, MA, 02115, USA.
Wentao LiangDepartment of Physics, Northeastern University, Boston, MA, 02115, USA.
Yongmin LiuDepartment of Electrical and Computer Engineering, Northeastern University, Boston, MA, 02115, USA.
Meni WanunuDepartment of Bioengineering, Northeastern University, Boston, MA, 02115, USA.ORCID https://orcid.org/0000-0002-9837-0004

Funding

Single-cell direct RNA sequencing using electrical zero-mode waveguides and engineered reverse transcriptasesR01HG011087 · NHGRI · NORTHEASTERN UNIVERSITY · PI WANUNU, MENI · 2020 to 2023
$3.0M
Direct picogram DNA and RNA sequencing using nanopore Zero-mode waveguidesR01HG009186 · NHGRI · NORTHEASTERN UNIVERSITY · PI WANUNU, MENI · 2016 to 2019
$2.3M
National Science Foundation ECCS-1542081NHGRI NIH HHS R01 HG009186NHGRI NIH HHS R01 HG011087NIH/National Human Genome Research Institute CBET-1931777NIH/National Human Genome Research Institute HG009186NIH/National Human Genome Research Institute HG011087
6 · The paper itself

Abstract

In contrast to sequence-specific techniques such as polymerase chain reaction, DNA sequencing does not require prior knowledge of the sample for surveying DNA. However, current sequencing technologies demand high inputs for a suitable library preparation, which typically necessitates DNA amplification, even for single-molecule sequencing methods. Here, electro-optical zero-mode waveguides (eZMWs) are presented, which can load DNA into the confinement of zero-mode waveguides with high efficiency and negligible DNA fragment length bias. Using eZMWs, highly efficient voltage-induced loading of DNA fragments of various sizes from ultralow inputs (nanogram-to-picogram levels) is observed. Rapid DNA fragment identification is demonstrated by burst sequencing of short and long DNA molecules (260 and 20 000 bp) loaded from an equimolar picomolar-level concentration mixture in just a few minutes. The device allows further studies in which low-input DNA capture is essential, for example, in epigenetics, where native DNA is required for obtaining modified base information.

Indexed as

DNANanotechnologyGene LibraryPolymerase Chain ReactionSequence Analysis, DNADNArapid DNA identificationsingle-molecule sequencingzero-mode waveguides

Identifiers

PMID34964522
PMCPMC8915919

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.