Evidence map›Paper›PMID 34985696›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Detection of HAC1 mRNA Splicing by RT-PCR in Saccharomyces cerevisiae.

Jagadeesh Kumar Uppala, Madhusudan Dey

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.2field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Disruption of thePathogens (Basel, Switzerland) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Jagadeesh Kumar UppalaDepartment of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI, USA.
Madhusudan DeyDepartment of Biological Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI, USA. deym@uwm.edu.
University of Wisconsin–Milwaukee · US

Funding

Regulating the Unfolded Protein Response in YeastR01GM124183 · NIGMS · UNIVERSITY OF WISCONSIN MILWAUKEE · PI DEY, MADHUSUDAN · 2017 to 2020
$1.3M
NIGMS NIH HHS R01 GM124183
6 · The paper itself

Abstract

HAC1 mRNA remains translationally repressed in the cytoplasm of the budding yeast Saccharomyces cerevisiae. Under conditions of cellular stress, a dual kinase RNase IRE1 (Inositol Requiring Enzyme-1) cleaves out an intervening sequence from the HAC1 mRNA. Cleaved mRNAs are then ligated by tRNA ligase, thus generating a spliced mRNA that translates an active transcription factor. This unconventional splicing of HAC1 mRNA in the cytoplasm is a molecular marker for various cellular stresses including oxidative stress and endoplasmic reticulum (ER) stress. This article describes a PCR-based protocol to detect the HAC1 mRNA splicing.

Indexed as

Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsBasic-Leucine Zipper Transcription FactorsGene Expression Regulation, FungalRepressor ProteinsReverse Transcriptase Polymerase Chain ReactionRNA, MessengerRNA SplicingBasic-Leucine Zipper Transcription FactorsRepressor ProteinsRNA, MessengerSaccharomyces cerevisiae ProteinsHAC1IRE1Protein foldingStress

Identifiers

PMID34985696
OpenAlexW4205892689

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.