ReviewAnnual review of biophysics2022
Protein Sequencing, One Molecule at a Time.
Review in Annual review of biophysics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 43 citations in OpenAlex.
- Large-scale single-molecule analysis of tau proteoforms.Nature methods · 2026Article
- Modulation of Single-Molecule Emission at Hexagonal Boron Nitride Surfaces.Nano letters · 2026Article
- Single-Cell Proteomic Technologies: Tools in the Quest for Principles.Annual review of biophysics · 2026Review
- Review
- Photolysis of the peptide bond at 193 and 222 nm.The Journal of chemical physics · 2025Article
- Paleoproteomics sheds light on million-year-old fossils.Nature reviews. Molecular cell biology · 2025Article
- A generalized protein identification method for novel and diverse sequencing technologies.NAR genomics and bioinformatics · 2024Article
- Estimating error rates for single molecule protein sequencing experiments.PLoS computational biology · 2024Article
- Analytical approaches for assessing protein structure in protein-rich food: A comprehensive review.Food chemistry: X · 2024Review
- Topographic fingerprinting of single proteins and proteoforms.Nature nanotechnology · 2024Article
- Full-length single-molecule protein fingerprinting.Nature nanotechnology · 2024Article
- Biological factors and statistical limitations prevent detection of most noncanonical proteins by mass spectrometry.PLoS biology · 2023Article
- Robust and scalable single-molecule protein sequencing with fluorosequencing.bioRxiv : the preprint server for biology · 2023Article
- Single-molecule fluorescence methods for protein biomarker analysis.Analytical and bioanalytical chemistry · 2023Review
- Unlocking the Power of Nanopores: Recent Advances in Biosensing Applications and Analog Front-End.Biosensors · 2023Review
- Amino acid sequence assignment from single molecule peptide sequencing data using a two-stage classifier.PLoS computational biology · 2023Article
- Uncovering biology by single-cell proteomics.Communications biology · 2023Review
- Cell-Free Production Systems in Droplet Microfluidics.Advances in biochemical engineering/biotechnology · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Despite tremendous gains over the past decade, methods for characterizing proteins have generally lagged behind those for nucleic acids, which are characterized by extremely high sensitivity, dynamic range, and throughput. However, the ability to directly characterize proteins at nucleic acid levels would address critical biological challenges such as more sensitive medical diagnostics, deeper protein quantification, large-scale measurement, and discovery of alternate protein isoforms and modifications and would open new paths to single-cell proteomics. In response to this need, there has been a push to radically improve protein sequencing technologies by taking inspiration from high-throughput nucleic acid sequencing, with a particular focus on developing practical methods for single-molecule protein sequencing (SMPS). SMPS technologies fall generally into three categories: sequencing by degradation (e.g., mass spectrometry or fluorosequencing), sequencing by transit (e.g., nanopores or quantum tunneling), and sequencing by affinity (as in DNA hybridization-based approaches). We describe these diverse approaches, which range from those that are already experimentally well-supported to the merely speculative, in this nascent field striving to reformulate proteomics.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.