Evidence mapPaperPMID 35008583Full record

ArticleInternational journal of molecular sciences2021

Localization and Absolute Quantification of Dopamine in Discrete Intravesicular Compartments Using NanoSIMS Imaging.

Stefania Rabasco, Tho D K Nguyen, Chaoyi Gu, Michael E Kurczy, Nhu T N Phan, Andrew G Ewing

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Forced polarisation of microglia by IL-13 is modified by inflammatory and microenvironmental context.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2025
    Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Spatial pharmacology using mass spectrometry imaging.Trends in pharmacological sciences · 2024
    Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Stefania RabascoDepartment of Chemistry and Molecular Biology, University of Gothenburg, SE-412 96 Gothenburg, Sweden.ORCID 0000-0001-5260-2909
Tho D K NguyenDepartment of Chemistry and Molecular Biology, University of Gothenburg, SE-412 96 Gothenburg, Sweden.ORCID 0000-0002-8306-6181
Chaoyi GuDepartment of Chemistry and Molecular Biology, University of Gothenburg, SE-412 96 Gothenburg, Sweden.
Michael E KurczyDrug Metabolism and Pharmacokinetics, Research and Early Development, Cardiovascular, Renal and Metabolism (CVRM), BioPharmaceuticals R&D, AstraZeneca, SE-431 83 Mölndal, Sweden.
Nhu T N PhanDepartment of Chemistry and Molecular Biology, University of Gothenburg, SE-412 96 Gothenburg, Sweden.
Andrew G EwingDepartment of Chemistry and Molecular Biology, University of Gothenburg, SE-412 96 Gothenburg, Sweden.ORCID 0000-0002-2084-0133
University of Gothenburg · SEAstraZeneca (Sweden) · SE

Funding

European Research Council 787534Knut and Alice Wallenberg Foundation NoneSwedish Research Council 2017-04366
6 · The paper itself

Abstract

The absolute concentration and the compartmentalization of analytes in cells and organelles are crucial parameters in the development of drugs and drug delivery systems, as well as in the fundamental understanding of many cellular processes. Nanoscale secondary ion mass spectrometry (NanoSIMS) imaging is a powerful technique which allows subcellular localization of chemical species with high spatial and mass resolution, and high sensitivity. In this study, we combined NanoSIMS imaging with spatial oversampling with transmission electron microscopy (TEM) imaging to discern the compartments (dense core and halo) of large dense core vesicles in a model cell line used to study exocytosis, and to localize

Indexed as

AnimalsCell Line, TumorDense Core VesiclesDopamineExocytosisMicroscopy, Electron, TransmissionOrganellesPC12 CellsRatsSpectrometry, Mass, Secondary IonDopamineabsolute quantificationdense core vesiclesdopamineNanoSIMSvesicular compartmentalization

Identifiers

PMID35008583
PMCPMC8745556
OpenAlexW4200552302

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.