Evidence map›Paper›PMID 35013106›Full record

ArticleCell death & disease2022

METTL14 suppresses pyroptosis and diabetic cardiomyopathy by downregulating TINCR lncRNA.

Liping Meng, Hui Lin, Xingxiao Huang, Jingfan Weng, Fang Peng, Shengjie Wu

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in Cell death & disease, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 115 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
115citing papers in PubMed, 1 pooled it
13.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

115 citing papers in PubMed, 1 synthesis or guideline pooled it, 170 citations in OpenAlex.

  1. Pooled it
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  18. PANoptosis in diabetes: immunometabolic insights and treatments.Apoptosis : an international journal on programmed cell death · 2026
    Review
  19. Mechanistic insights into PMEnvironmental epigenetics · 2026
    Article
  20. Review

55 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Liping Meng *Department of Cardiology, Shaoxing People's Hospital(Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, 312000, Zhejiang, China.ORCID http://orcid.org/0000-0002-6139-2031
Hui Lin *Department of Cardiology, Shaoxing People's Hospital(Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, 312000, Zhejiang, China.ORCID http://orcid.org/0000-0001-5742-6799
Xingxiao HuangDepartment of Cardiology, Shaoxing People's Hospital(Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, 312000, Zhejiang, China.
Jingfan WengDepartment of Cardiology, Shaoxing People's Hospital(Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, 312000, Zhejiang, China.
Fang PengDepartment of Cardiology, Shaoxing People's Hospital(Shaoxing Hospital, Zhejiang University School of Medicine), Shaoxing, 312000, Zhejiang, China. sxrmyypf@126.com.ORCID http://orcid.org/0000-0002-3826-5632
Shengjie WuDepartment of Cardiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. wusj120@163.com.ORCID http://orcid.org/0000-0002-8981-9789
Shaoxing People's Hospital · CNWenzhou Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

N6-methyladenosine (m6A) is one of the most important epigenetic regulation of RNAs, such as lncRNAs. However, the underlying regulatory mechanism of m6A in diabetic cardiomyopathy (DCM) is very limited. In this study, we sought to define the role of METTL14-mediated m6A modification in pyroptosis and DCM progression. DCM rat model was established and qRT-PCR, western blot, and immunohistochemistry (IHC) were used to detect the expression of METTL14 and TINCR. Gain-and-loss functional experiments were performed to define the role of METTL14-TINCR-NLRP3 axis in pyroptosis and DCM. RNA pulldown and RNA immunoprecipitation (RIP) assays were carried out to verify the underlying interaction. Our results showed that pyroptosis was tightly involved in DCM progression. METTL14 was downregulated in cardiomyocytes and hear tissues of DCM rat tissues. Functionally, METTL14 suppressed pyroptosis and DCM via downregulating lncRNA TINCR, which further decreased the expression of key pyroptosis-related protein, NLRP3. Mechanistically, METTL14 increased m6A methylation level of TINCR gene, resulting in its downregulation. Moreover, the m6A reader protein YTHDF2 was essential for m6A methylation and mediated the degradation of TINCR. Finally, TINCR positively regulated NLRP3 by increasing its mRNA stability. To conclude, our work revealed the novel role of METTL14-mediated m6A methylation and lncRNA regulation in pyroptosis and DCM, which could help extend our understanding the epigenetic regulation of pyroptosis in DCM progression.

Indexed as

PyroptosisAdenosineAnimalsDiabetic CardiomyopathiesDown-RegulationEpigenesis, GeneticMethylationMethyltransferasesMyocytes, CardiacNLR Family, Pyrin Domain-Containing 3 ProteinRatsRNA-Binding ProteinsRNA, Long NoncodingRNA StabilityAdenosineMethyltransferasesMettl14 protein, ratNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, ratN-methyladenosineRNA-Binding ProteinsRNA, Long Noncoding

Identifiers

PMID35013106
PMCPMC8748685
OpenAlexW4220892966

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.