ArticlePlant methods2022
Improving large-scale biomass and total alkaloid production of Dendrobium nobile Lindl. using a temporary immersion bioreactor system and MeJA elicitation.
Article in Plant methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Plant-derived extracellular vesicles quality control: key process progress and future research directions.Discover nano · 2025Review
- Enhanced bioproduction of phenolic compounds fromFrontiers in molecular biosciences · 2025Article
- Comparative Assessment of Lignan Profiling and Biological Activities ofPharmaceuticals (Basel, Switzerland) · 2024Article
- Orchid Micropropagation Using Temporary Immersion Systems: A Review.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Enhancing dendrobine production inFrontiers in plant science · 2024Article
- Micropropagation of Encyclia cordigera (Kunth) Dressler in Ebb-and-Flow Bioreactor.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Temporary Immersion System for Biomass Production of Salvia spp.: A Mini-Review.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review.Plants (Basel, Switzerland) · 2023Review
- Analysis of the response mechanisms ofFrontiers in plant science · 2023Article
- Transcriptome Analysis of Dendrobine Biosynthesis inFrontiers in microbiology · 2022Article
- Jasmonates in plant growth and development and elicitation of secondary metabolites: An updated overview.Frontiers in plant science · 2022Review
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDendrobium nobile Lindl. is an important pharmacopeial plant with medicinal and ornamental value. This study sought to provide a technical means for the large-scale production of total alkaloid in D. nobile. Seedlings were cultured in vitro using a temporary immersion bioreactor system (TIBS). The four tested immersion frequencies (min/h; 5/2, 5/4, 5/6, and 5/8) influenced the production of biomass and total alkaloid content. In addition, to compare the effects of different concentrations of the phytohormone methyl jasmonate (MeJA) and treatment time on biomass and total alkaloid accumulation, MeJA was added to the TIBS medium after 50 days. Finally, total alkaloid production in semi-solid system (SSS), TIBS, and TIBS combined with the MeJA system (TIBS-MeJA) were compared.
resultsThe best immersion frequency was found to be 5/6 (5 min every 6 h), which ensured appropriate levels of biomass and total alkaloid content in plantlets. The alkaloid content and production level of seedlings were the highest after treatment with 10 μM MeJA separately for 20 and 30 days using TIBS. The maximum content (7.41 mg/g DW) and production level (361.24 mg/L) of total alkaloid on use of TIBS-MeJA were 2.32- and 4.69-fold, respectively, higher in terms of content, and 2.07- and 10.49-fold, respectively, higher in terms of production level than those on using of TIBS (3.20 mg/g DW, 174.34 mg/L) and SSS (1.58 mg/g DW, 34.44 mg/L).
conclusionsOur results show TIBS-MeJA is suitable for large-scale production of total alkaloid in in vitro seedlings. Therefore, this study provides a technical means for the large-scale production of total alkaloid in D. nobile.
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