ArticleFrontiers in plant science2021
Modified "Allele-Specific qPCR" Method for SNP Genotyping Based on FRET.
Article in Frontiers in plant science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Article
- The Specific Low-Interference dsDNA Copper Nanoclusters for Visual Fluorescent Detection and Quantification of theAnalytical chemistry · 2026Article
- Comprehensive web-based platform for advanced PCR design, genotyping, synthetic biology, molecular diagnostics, and sequence analysis.Molecular therapy. Nucleic acids · 2025Article
- Genotyping ofPlants (Basel, Switzerland) · 2025Article
- Longer Internode with Same Cell Length:Plants (Basel, Switzerland) · 2025Article
- Development of a diagnostic variable number tandem repeat marker and dual TaqMan genotyping assay to distinguish Lophophora species.International journal of legal medicine · 2025Article
- An Assay for Variable Fragment Length Allele-Specific Genotyping.Methods in molecular biology (Clifton, N.J.) · 2025Article
- TAL-SRX: an intelligent typing evaluation method for KASP primers based on multi-model fusion.Frontiers in plant science · 2025Article
- Zinc finger knuckle genes are associated with tolerance to drought and dehydration in chickpea (Frontiers in plant science · 2024Article
- A FEN 1-driven DNA walker-like reaction coupling with magnetic bead-based separation for specific SNP detection.Frontiers in bioengineering and biotechnology · 2023Article
- Real-time allelic assays of SARS-CoV-2 variants to enhance sewage surveillance.Water research · 2022Article
- Designing Allele-Specific Competitive-Extension PCR-Based Assays for High-Throughput Genotyping and Gene Characterization.Frontiers in molecular biosciences · 2022Review
- Expression of Specific Alleles of Zinc-Finger Transcription Factors,International journal of molecular sciences · 2021Article
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13 authors.
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Abstract
The proposed method is a modified and improved version of the existing "Allele-specific q-PCR" (ASQ) method for genotyping of single nucleotide polymorphism (SNP) based on fluorescence resonance energy transfer (FRET). This method is similar to frequently used techniques like Amplifluor and Kompetitive allele specific PCR (KASP), as well as others employing common universal probes (UPs) for SNP analyses. In the proposed ASQ method, the fluorophores and quencher are located in separate complementary oligonucleotides. The ASQ method is based on the simultaneous presence in PCR of the following two components: an allele-specific mixture (allele-specific and common primers) and a template-independent detector mixture that contains two or more (up to four) universal probes (UP-1 to 4) and a single universal quencher oligonucleotide (Uni-Q). The SNP site is positioned preferably at a penultimate base in each allele-specific primer, which increases the reaction specificity and allele discrimination. The proposed ASQ method is advanced in providing a very clear and effective measurement of the fluorescence emitted, with very low signal background-noise, and simple procedures convenient for customized modifications and adjustments. Importantly, this ASQ method is estimated as two- to ten-fold cheaper than Amplifluor and KASP, and much cheaper than all those methods that rely on dual-labeled probes without universal components, like TaqMan and Molecular Beacons. Results for SNP genotyping in the barley genes
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