ArticleJournal of biomedical materials research. Part A2022
Evaluation of novel biomaterials for cartilage regeneration based on gelatin methacryloyl interpenetrated with extractive chondroitin sulfate or unsulfated biotechnological chondroitin.
Article in Journal of biomedical materials research. Part A, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Engineering Nanoscale Frontiers: Valve Metal Oxide Nanostructures From Fundamentals to Multifunctional Biomedical Applications.Chemical record (New York, N.Y.) · 2026Review
- Chondrogenic potential of PMSCs cultured on chondroitin sulfate/gelatin-modified DBM scaffold.BioImpacts : BI · 2025Article
- Influence of Gelatin on Adhesion, Proliferation, and Adipogenic Differentiation of Adipose Tissue-Derived Stem Cells Cultured on Soy Protein-Agarose Scaffolds.Foods (Basel, Switzerland) · 2024Article
- Chondrogenic potential of superficial versus cartilage layer cells of the temporomandibular joint condyle in photopolymerizable gelatin-based hydrogels.Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine · 2024Article
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- Article
- Involvement of Yes-Associated Protein 1 Activation in the Matrix Degradation of Human-Induced-Pluripotent-Stem-Cell-Derived Chondrocytes Induced by T-2 Toxin and Deoxynivalenol Alone and in Combination.International journal of molecular sciences · 2024Article
- Adult Mesenchymal Stem Cells in Presence of Glycosaminoglycans.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Article
- GelMA synthesis and sources comparison for 3D multimaterial bioprinting.Frontiers in bioengineering and biotechnology · 2024Article
- Hyaluronic Acid-Based Injective Medical Devices: In Vitro Characterization of Novel Formulations Containing Biofermentative Unsulfated Chondroitin or Extractive Sulfated One with Cyclodextrins.Pharmaceuticals (Basel, Switzerland) · 2023Article
- Review
- Clinical Trials with Mesenchymal Stem Cell Therapies for Osteoarthritis: Challenges in the Regeneration of Articular Cartilage.International journal of molecular sciences · 2023Review
- 3D Printing Technology Based on Versatile Gelatin-Carrageenan Gel System for Drug Formulations.Pharmaceutics · 2023Article
- Properties and Printability of the Synthesized Hydrogel Based on GelMA.International journal of molecular sciences · 2023Article
- Molecular Fingerprint of Human Pathological Synoviocytes in Response to Extractive Sulfated and Biofermentative Unsulfated Chondroitins.International journal of molecular sciences · 2022Article
- Recent Advances in Smart Hydrogels Prepared by Ionizing Radiation Technology for Biomedical Applications.Polymers · 2022Review
- Review
- Novel Polyurethane Scaffolds Containing Sucrose Crosslinker for Dental Application.International journal of molecular sciences · 2022Article
- Evaluation of novel biomaterials for cartilage regeneration based on gelatin methacryloyl interpenetrated with extractive chondroitin sulfate or unsulfated biotechnological chondroitin.Journal of biomedical materials research. Part A · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Gelatin is widely proposed as scaffold for cartilage tissue regeneration due to its high similarities to the extracellular matrix. However, poor mechanical properties and high sensitivity to enzymatic degradation encouraged the scientific community to develop strategies to obtain better performing hydrogels. Gelatin networks, specifically gelatin-methacryloyl (GM), have been coupled to hyaluronan or chondroitin sulfate (CS). In this study, we evaluated the biophysical properties of an innovative photocross-linked hydrogel based on GM with the addition of CS or a new unsulfated biotechnological chondroitin (BC). Biophysical, mechanical, and biochemical characterization have been assessed to compare GM hydrogels to the chondroitin containing networks. Moreover, mesenchymal stem cells (MSCs) were seeded on these biomaterials in order to evaluate the differentiation toward the chondrocyte phenotype in 21 days. Rheological characterization showed that both CS and BC increased the stiffness (G' was about 2-fold), providing a stronger rigid matrix, with respect to GM alone. The biological tests confirmed the onset of MSCs differentiation process starting from 14 days of in vitro culture. In particular, the combination GM + BC resulted to be more effective than GM + CS in the up-regulation of key genes such as collagen type 2A1 (COLII), SOX-9, and aggrecan). In addition, the scanning microscope analyses revealed the cellular adhesion on materials and production of extracellular vesicles. Immunofluorescence staining confirmed an increase of COLII in presence of both chondroitins. Finally, the outcomes suggest that BC entangled within cross-linked GM matrix may represent a promising new biomaterial with potential applications in cartilage regeneration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.