Evidence map›Paper›PMID 35127859›Full record

ArticleFrontiers in cardiovascular medicine2021

Andrographolide Promotes Interaction Between Endothelin-Dependent EDNRA/EDNRB and Myocardin-SRF to Regulate Pathological Vascular Remodeling.

Wangming Hu, Xiao Wu, Zhong Jin, Zheng Wang, Qiru Guo, Zixian Chen, Song Zhu, Haidi Zhang, Jian Huo, Lingling Zhang and 5 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in cardiovascular medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
4.0field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.

  1. Dissecting the Genetic Architecture of Intracranial Aneurysms.Circulation. Genomic and precision medicine · 2025
    Pooled it
  2. Article
  3. Article
  4. Loss ofAmerican journal of respiratory cell and molecular biology · 2025
    Article
  5. Review
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  7. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Wangming HuCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Xiao WuCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Zhong JinCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Zheng WangCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Qiru GuoCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Zixian ChenSchool of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Song ZhuHospital of Chengdu University of Traditional Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Haidi ZhangHospital of Chengdu University of Traditional Chinese Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Jian HuoChengdu Women's and Children's Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, China.
Lingling ZhangCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Xin ZhouCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Lan YangCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Huan XuCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Liangqing ShiCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Yong WangCollege of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Chengdu University of Traditional Chinese Medicine · CNChengdu Women's and Children's Central Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionPathological vascular remodeling is a hallmark of various vascular diseases. Smooth muscle cell (SMC) phenotypic switching plays a pivotal role during pathological vascular remodeling. The mechanism of how to regulate SMC phenotypic switching still needs to be defined. This study aims to investigate the effect of Andrographolide, a key principle isolated from Andrographis paniculate, on pathological vascular remodeling and its underlying mechanism.

methodsA C57/BL6 mouse left carotid artery complete ligation model and rat SMCs were used to determine whether Andrographolide is critical in regulating SMC phenotypic switching. Quantitative real-time PCR, a CCK8 cell proliferation assay, BRDU incorporation assay, Boyden chamber migration assay, and spheroid sprouting assay were performed to evaluate whether Andrographolide suppresses SMC proliferation and migration. Immunohistochemistry staining, immunofluorescence staining, and protein co-immunoprecipitation were used to observe the interaction between EDNRA, EDNRB, and Myocardin-SRF.

resultsAndrographolide inhibits neointimal hyperplasia in the left carotid artery complete ligation model. Andrographolide regulates SMC phenotypic switching characterized by suppressing proliferation and migration. Andrographolide activates the endothelin signaling pathway exhibited by dramatically inducing EDNRA and EDNRB expression. The interaction between EDNRA/EDNRB and Myocardin-SRF resulted in promoting SMC differentiation marker gene expression.

conclusionAndrographolide plays a critical role in regulating pathological vascular remodeling.

Indexed as

AndrographolideCArG boxEDNRAEDNRBMyocardin-SRFpathological vascular remodeling

Identifiers

PMID35127859
PMCPMC8810813
OpenAlexW4206700258

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.