Evidence map›Paper›PMID 35157090›Full record

ArticleInflammation research : official journal of the European Histamine Research Society ... [et al.]2022

Cytokine producing ability of peripheral blood cells from COVID-19 patients after unspecific in vitro stimulation.

Snezana Zivancevic-Simonovic, Danijela Jovanovic, Vojislav Cupurdija, Olivera Milosevic-Djordjevic, Marijana Stanojevic, Milos Marinkovic, Nebojsa Igrutinovic, Ivan Stanojevic, Danilo Vojvodic, Olgica Mihaljevic

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Snezana Zivancevic-SimonovicDepartment of Pathophysiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia. snezana@medf.kg.ac.rs.ORCID http://orcid.org/0000-0002-2400-3850
Danijela JovanovicDepartment of Internal Medicine, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.ORCID http://orcid.org/0000-0002-9421-4178
Vojislav CupurdijaDepartment of Internal Medicine, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Olivera Milosevic-DjordjevicDepartment of Genetics, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.ORCID http://orcid.org/0000-0002-5049-135X
Marijana StanojevicUniversity Clinical Center Kragujevac, Kragujevac, Serbia.ORCID http://orcid.org/0000-0002-1068-777X
Milos MarinkovicUniversity Clinical Center Kragujevac, Kragujevac, Serbia.
Nebojsa IgrutinovicUniversity Clinical Center Kragujevac, Kragujevac, Serbia.
Ivan StanojevicInstitute for Medical Research, Military Medical Academy, Belgrade, Serbia.
Danilo VojvodicInstitute for Medical Research, Military Medical Academy, Belgrade, Serbia.ORCID http://orcid.org/0000-0001-9308-3076
Olgica MihaljevicDepartment of Pathophysiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovica 69, 34000, Kragujevac, Serbia.ORCID http://orcid.org/0000-0002-5614-210X
University of Kragujevac · RSClinical Centre of Kragujevac · RSMilitary Medical Academy · RS

Funding

Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja III41010Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja ON175069
6 · The paper itself

Abstract

OBJECTIVE AND

designPerturbations of peripheral T cell homeostasis and dysregulation of the immune response to SARS-CoV-2, especially in severely ill patients, were observed. The aim of this study was to analyze the cytokine producing ability of peripheral blood cells from severely ill COVID-19 patients upon non-specific in vitro stimulation with phytohemagglutinin (PHA). Possible associations of cytokine levels with patients' age and gender, glucocorticosteroid therapy, as well as the trend of the inflammatory process at the time of sampling (increased or decreased) were also analyzed. SUBJECTS AND

methodsThe study included 23 COVID-19 patients and 17 healthy control subjects. The concentrations of selected Th1/Th2/Th9/Th17/Th22 cytokines were determined using a multi-analyte flow assay kit.

resultsOur results showed that peripheral blood cells from severely ill COVID-19 patients had a much reduced ability to produce cytokines in comparison to healthy controls. When inflammation was raised, blood cells produced more IL-6 and IL-17, which led to increases of some Th17/Th1 and Th17/Th2 ratios, skewing towards the Th17 type of response. The methylprednisolone used in the treatment of patients with COVID-19 influences the production of several cytokines in dose dependent manner.

conclusionOur results indicate that the stage of the inflammatory process at the time of sampling and the dose of the applied glucocorticosteroid therapy might influence cytokine producing ability upon non-specific stimulation of T cells in vitro.

Indexed as

SARS-CoV-2AdultAgedAnti-Inflammatory AgentsBlood CellsCells, CulturedCOVID-19COVID-19 Drug TreatmentCytokinesFemaleGlucocorticoidsHumansMaleMethylprednisoloneMiddle AgedMitogensAnti-Inflammatory AgentsCytokinesGlucocorticoidsMethylprednisoloneMitogensPhytohemagglutininsCOVID-19CytokineGlucocorticosteroidInflammationIn vitroPhytohemagglutinin

Identifiers

PMID35157090
PMCPMC8853300
OpenAlexW4213122584

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.