Evidence map›Paper›PMID 35186663›Full record

Article3 Biotech2022

Knockdown of capsid protein encoding novel ATPase domain inhibits genome packaging in potato leafroll virus.

Jitesh Kumar, Ravi Ranjan Kumar, Dilip Kumar Das, Auroshikha Mohanty, Kumari Rajani, Namaste Kumari, Vinod Kumar, Sunil Kumar, Bajarang Vasant Kumbhar, Tushar Ranjan

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in 3 Biotech, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.3field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Jitesh Kumar *Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur, Bihar India.
Ravi Ranjan Kumar *Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur, Bihar India.
Dilip Kumar DasDepartment of Biotechnology, Tilka Manjhi Bhagalpur University, Bhagalpur, Bihar India.
Auroshikha MohantyDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur, Bihar India.
Kumari RajaniDepartment of Seed Science and Technology, Bihar Agricultural University, Sabour, Bhagalpur, Bihar India.
Namaste KumariDepartment of Plant Physiology, Govind Ballabh Pant University of Agriculture and Technology, Pantnagar, Uttrakhand India.
Vinod KumarDepartment of Molecular Biology and Genetic Engineering, Pulse Research Centre, Mokama, Bihar India.
Sunil KumarKusuma School of Biological Sciences, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, India.
Bajarang Vasant KumbharDepartment of Biological Sciences, Sunandan Divatia School of Science, NMIMS University, Vile Parle, Mumbai, India.
Tushar RanjanDepartment of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, Bhagalpur, Bihar India.ORCID 0000-0002-6805-7201
Bihar Agricultural University · INGovind Ballabh Pant University of Agriculture and Technology · INIndian Institute of Technology Delhi · INNarsee Monjee Institute of Management Studies · INTilka Manjhi Bhagalpur University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Potato leafroll virus (PLRV) uses powerful molecular machines to package its genome into a viral capsid employing ATP as fuel. Although, recent bioinformatics and structural studies have revealed detailed mechanism of DNA packaging, little is known about the mechanochemistry of genome packaging in small plant viruses such as PLRV. We have identified a novel P-loop-containing ATPase domain with two Walker A-like motifs, two arginine fingers, and two sensor motifs distributed throughout the polypeptide chain of PLRV capsid protein (CP). The composition and arrangement of the ATP binding and hydrolysis domain of PLRV CP is unique and rarely reported. The discovery of the system sheds new light on the mechanism of viral genome packaging, regulation of viral assembly process, and evolution of plant viruses. Here, we used the RNAi approach to suppress CP gene expression, which in turn prevented PLRV genome packaging and assembly in

Indexed as

Capsid proteinGenome packagingPackaging motorPlant virusP-loop-containing ATPase domainRNA interference

Identifiers

PMID35186663
PMCPMC8831665
OpenAlexW4211180485

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.