Evidence mapPaperPMID 35196979Full record

ArticleBMC cardiovascular disorders2022

Rosuvastatin exerts cardioprotective effect in lipopolysaccharide-mediated injury of cardiomyocytes in an MG53-dependent manner.

Jiawei Zhuang, Gangyi Cheng, Jian Huang, Hongwei Guo, Yiquan Lai, Jiamao Wang, Zhonggui Shan, Shaoyi Zheng

Open access · goldAbstract read
In one paragraph

Article in BMC cardiovascular disorders, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Jiawei ZhuangDepartment of Cardiovascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China.
Gangyi ChengDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Jian HuangDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Hongwei GuoDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Yiquan LaiDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Jiamao WangDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Zhonggui ShanDepartment of Cardiovascular Surgery, The First Affiliated Hospital of Xiamen University, Xiamen, China. szgdoctor@126.com.
Shaoyi ZhengDepartment of Cardiovascular Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, China. shaoyizheng@yahoo.com.
First Affiliated Hospital of Xiamen University · CNSouthern Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMyocarditis is a cardiomyopathy associated with the inflammatory response. Rosuvastatin (RS) demonstrates cardioprotective effect in the clinical setting, although its cellular and molecular mechanisms in ameliorating myocarditis are largely unknown. MG53 (muscle-specific E3 ligase Mitsugumin 53), a newly identified striated muscle-specific protein, is involved in skeletal muscle membrane repair. We aimed to explore whether RS mediated the repair of cardiomyocytes in an MG53-dependent manner.

methodsThe RS-induced upregulation of MG53 was determined using RT-qPCR and western blotting. A lipopolysaccharide (LPS)-induced cell inflammatory model was constructed using rat cardiac muscle cell H9C2. Inflammatory injury was evaluated according to the alterations of cell viability, mitochondrial membrane potential, cell apoptosis, and expression of pro-inflammatory cytokines (interleukin-1β, interleukin-6, tumor necrosis factor-α, and monocyte chemoattractant protein-1). Small interfering RNAs (siRNAs) were used to silence MG53. The cardioprotective effect of RS and the inhibition of this protection by MG53 silence were evaluated in the forementioned in vitro model. The underlying mechanism was finally investigated using western blotting to detected the expressions of apoptotic markers (Bcl-2, Bax, Cleaved caspase-9, Cleaved caspase-3), cell cycle regulatory factors (Cyclin A, Cyclin E1, Cyclin D1, CDK2), and components involved in NF-κB signaling pathway (p-IκBa, Iκba, p-p65, p65).

resultsRS ameliorated LPS-induced inflammatory injury. RS upregulated the expression of MG53. MG53 was crucial for the RS-mediated repair response in vitro. Ablation of MG53 inhibited the RS-mediated protective effect. Furthermore, RS and MG53 interact in multiple signaling pathways to modulate recovery.

conclusionRS exerts cardioprotective effect in an MG53-dependent manner. MG53 may serve as a novel drug target for myocarditis treatment.

Indexed as

LipopolysaccharidesMyocarditisAnimalsHumansMuscle ProteinsMyocytes, CardiacNF-kappa BRatsRosuvastatin CalciumLipopolysaccharidesMuscle ProteinsNF-kappa BRosuvastatin CalciumMyocarditisRosuvastatin MG53

Identifiers

PMID35196979
PMCPMC8865731
OpenAlexW4214562813

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.