ArticleInternational journal of molecular sciences2022
Investigation of Metabolome Underlying the Biological Mechanisms of Acute Heat Stressed Granulosa Cells.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Metabolomic profiling delineates the role of adenosine in oocyte quality and embryonic development.Cell death & disease · 2026Article
- Multi-omics dissection of heat stress reveals CIITA as a central regulator of metabolic thermotolerance in cattle (Bos taurus).Communications biology · 2026Article
- Heat Shock Affects Amino Acid Metabolism in Bovine Cumulus Cells and Denuded Oocytes During In Vitro Maturation.Biology · 2026Article
- Effects of different seasons on the outcome ofFrontiers in reproductive health · 2026Article
- Fisetinidin chloride ameliorates carbon tetrachloride-induced hepatotoxicity in HepaRG cells.Journal of bioenergetics and biomembranes · 2025Article
- Integrated Metabolomic and Transcriptomic Analysis Decodes Heat Stress-Induced Metabolic Shifts in Gilt Granulosa Cells.Veterinary sciences · 2025Article
- An Overview of Advancements in Proteomic Approaches to Enhance Livestock Production and Aquaculture.Animals : an open access journal from MDPI · 2025Review
- Integrated Multi-Omics Analysis Reveals the Mechanisms of Intestinal Cell Injury Under Different Levels of Heat Stress.International journal of molecular sciences · 2025Article
- Proteomic identification of potential biomarkers for heat tolerance in Caracu beef cattle using high and low thermotolerant groups.BMC genomics · 2024Article
- Advances in the Effects of Heat Stress on Ovarian Granulosa Cells: Unveiling Novel Ferroptosis Pathways.Veterinary sciences · 2024Review
- Vitamin C Alleviates the Negative Effects of Heat Stress on Reproductive Processes by Regulating Amino Acid Metabolism in Granulosa Cells.Antioxidants (Basel, Switzerland) · 2024Article
- Incorporation of L-Carnitine in the OvSynch protocol enhances the morphometrical and hemodynamic parameters of the ovarian structures and uterus in ewes under summer climatic conditions.BMC veterinary research · 2023Article
- Analysis of CircRNA Expression in Peripheral Blood of Holstein Cows in Response to Heat Stress.International journal of molecular sciences · 2023Article
- Plasma metabolomic characterization of premature ovarian insufficiency.Journal of ovarian research · 2023Observational
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- Integrated analysis of the expression profiles of the lncRNA-miRNA-mRNA ceRNA network in granulosa and cumulus cells from yak ovaries.BMC genomics · 2022Article
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Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Heat stress affects granulosa cells and the ovarian follicular microenvironment, ultimately resulting in poor oocyte developmental competence. This study aims to investigate the metabo-lomics response of bovine granulosa cells (bGCs) to in vitro acute heat stress of 43 °C. Heat stress triggers oxidative stress-mediated apoptosis in cultured bGCs. Heat-stressed bGCs exhibited a time-dependent recovery of proliferation potential by 48 h. A total of 119 metabolites were identified through LC-MS/MS-based metabolomics of the spent culture media, out of which, 37 metabolites were determined as differentially involved in metabolic pathways related to bioenergetics support mechanisms and the physical adaptations of bGCs. Multiple analyses of metabolome data identified choline, citric acid, 3-hydroxy-3-methylglutaric acid, glutamine, and glycocyamine as being upregulated, while galactosamine, AICAR, ciliatine, 16-hydroxyhexadecanoic acid, lysine, succinic acid, uridine, xanthine, and uraconic acid were the important downregulated metabolites in acute heat stress. These differential metabolites were implicated in various important metabolic pathways directed towards bioenergetics support mechanisms including glycerophospholipid metabolism, the citrate cycle (TCA cycle), glyoxylate and dicarboxylate metabolism, and serine, threonine, and tyrosine metabolism. Our study presents important metabolites and metabolic pathways involved in the adaptation of bGCs to acute heat stress in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.