ArticlePLoS pathogens2022
Single-cell transcriptomics reveals expression profiles of Trypanosoma brucei sexual stages.
Article in PLoS pathogens, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- High-resolution single-cell sequencing of trans-spliced mRNA.Nature protocols · 2026Review
- Single-cell RNA-seq reveals trans-sialidase-like superfamily gene expression heterogeneity ineLife · 2026Article
- Next generation genetic screens in kinetoplastids.Nucleic acids research · 2025Review
- Single-cell atlas ofProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Advances in the Immunology of the Host-Parasite Interactions in African Trypanosomosis, including Single-Cell Transcriptomics.Pathogens (Basel, Switzerland) · 2024Review
- A sticky situation: When trypanosomatids attach to insect tissues.PLoS pathogens · 2023Review
- Differential expression of meiosis and homologous recombination-related genes in the life cycle of Trypanosoma cruzi.Parasitology research · 2023Article
- Article
- Transcriptomic analysis of the adaptation to prolonged starvation of the insect-dwellingFrontiers in cellular and infection microbiology · 2023Article
- A long noncoding RNA promotes parasite differentiation in African trypanosomes.Science advances · 2022Article
- Signatures of hybridization in Trypanosoma brucei.PLoS pathogens · 2022Article
- Paving the Way: Contributions of Big Data to Apicomplexan and Kinetoplastid Research.Frontiers in cellular and infection microbiology · 2022Review
- Parasite OMICS, the grand challenges ahead.Frontiers in parasitology · 2022Article
- Sex in protists: A new perspective on the reproduction mechanisms of trypanosomatids.Genetics and molecular biology · 2022Article
- Unraveling the intricacies of host-pathogen interaction through single-cell genomics.Microbes and infectionReview
- Life cycle strategies in free-living unicellular eukaryotes: Diversity, evolution, and current molecular tools to unravel the private life of microorganisms.The Journal of eukaryotic microbiologyReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Early diverging lineages such as trypanosomes can provide clues to the evolution of sexual reproduction in eukaryotes. In Trypanosoma brucei, the pathogen that causes Human African Trypanosomiasis, sexual reproduction occurs in the salivary glands of the insect host, but analysis of the molecular signatures that define these sexual forms is complicated because they mingle with more numerous, mitotically-dividing developmental stages. We used single-cell RNA-sequencing (scRNAseq) to profile 388 individual trypanosomes from midgut, proventriculus, and salivary glands of infected tsetse flies allowing us to identify tissue-specific cell types. Further investigation of salivary gland parasite transcriptomes revealed fine-scale changes in gene expression over a developmental progression from putative sexual forms through metacyclics expressing variant surface glycoprotein genes. The cluster of cells potentially containing sexual forms was characterized by high level transcription of the gamete fusion protein HAP2, together with an array of surface proteins and several genes of unknown function. We linked these expression patterns to distinct morphological forms using immunofluorescence assays and reporter gene expression to demonstrate that the kinetoplastid-conserved gene Tb927.10.12080 is exclusively expressed at high levels by meiotic intermediates and gametes. Further experiments are required to establish whether this protein, currently of unknown function, plays a role in gamete formation and/or fusion.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.