Evidence mapPaperPMID 35255604Full record

ArticleEndocrinology and metabolism (Seoul, Korea)2022

EW-7197 Attenuates the Progression of Diabetic Nephropathy in db/db Mice through Suppression of Fibrogenesis and Inflammation.

Kyung Bong Ha, Weerapon Sangartit, Ah Reum Jeong, Eun Soo Lee, Hong Min Kim, Soyeon Shim, Upa Kukongviriyapan, Dae-Kee Kim, Eun Young Lee, Choon Hee Chung

Open access · diamondAbstract read
In one paragraph

Article in Endocrinology and metabolism (Seoul, Korea), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 11 citations in OpenAlex.

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  8. TGF-β signaling in health, disease, and therapeutics.Signal transduction and targeted therapy · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Kyung Bong HaDepartment of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
Weerapon SangartitDepartment of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Ah Reum JeongDepartment of Internal Medicine and Institute of Tissue Regeneration, Soonchunhyang University College of Medicine, Cheonan, Korea.
Eun Soo LeeDepartment of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
Hong Min KimDepartment of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
Soyeon ShimDepartment of Pharmacy, Ewha Womans University College of Pharmacy, Seoul, Korea.
Upa KukongviriyapanDepartment of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Dae-Kee KimDepartment of Pharmacy, Ewha Womans University College of Pharmacy, Seoul, Korea.
Eun Young LeeDepartment of Internal Medicine and Institute of Tissue Regeneration, Soonchunhyang University College of Medicine, Cheonan, Korea.
Choon Hee ChungDepartment of Internal Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea.
Yonsei University · KREwha Womans University · KRKhon Kaen University · THSoonchunhyang University · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDiabetic nephropathy (DN) is characterized by albuminuria and accumulation of extracellular matrix (ECM) in kidney. Transforming growth factor-β (TGF-β) plays a central role in promoting ECM accumulation. We aimed to examine the effects of EW-7197, an inhibitor of TGF-β type 1 receptor kinase (ALK5), in retarding the progression of DN, both in vivo, using a diabetic mouse model (db/db mice), and in vitro, in podocytes and mesangial cells.

methodsIn vivo study: 8-week-old db/db mice were orally administered EW-7197 at a dose of 5 or 20 mg/kg/day for 10 weeks. Metabolic parameters and renal function were monitored. Glomerular histomorphology and renal protein expression were evaluated by histochemical staining and Western blot analyses, respectively. In vitro study: DN was induced by high glucose (30 mM) in podocytes and TGF-β (2 ng/mL) in mesangial cells. Cells were treated with EW-7197 (500 nM) for 24 hours and the mechanism associated with the attenuation of DN was investigated.

resultsEnhanced albuminuria and glomerular morphohistological changes were observed in db/db compared to that of the nondiabetic (db/m) mice. These alterations were associated with the activation of the TGF-β signaling pathway. Treatment with EW-7197 significantly inhibited TGF-β signaling, inflammation, apoptosis, reactive oxygen species, and endoplasmic reticulum stress in diabetic mice and renal cells.

conclusionEW-7197 exhibits renoprotective effect in DN. EW-7197 alleviates renal fibrosis and inflammation in diabetes by inhibiting downstream TGF-β signaling, thereby retarding the progression of DN. Our study supports EW-7197 as a therapeutically beneficial compound to treat DN.

Indexed as

Diabetes Mellitus, ExperimentalDiabetic NephropathiesAniline CompoundsAnimalsInflammationMiceTriazolesAniline CompoundsTriazolesvactosertibActivin receptor-like kinase 5Diabetic nephropathiesGlomerular mesangial cellsPodocytesTransforming growth factor beta

Identifiers

PMID35255604
PMCPMC8901963
OpenAlexW4214599697

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.