Evidence map›Paper›PMID 35262485›Full record

ArticleParasite (Paris, France)2022

TbKINX1B: a novel BILBO1 partner and an essential protein in bloodstream form Trypanosoma brucei.

Doranda Perdomo, Elodie Berdance, Gertrud Lallinger-Kube, Annelise Sahin, Denis Dacheux, Nicolas Landrein, Anne Cayrel, Klaus Ersfeld, Mélanie Bonhivers, Linda Kohl and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite (Paris, France), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Doranda PerdomoUniversity of Bordeaux, CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, 33000 Bordeaux, France.ORCID 0000-0001-9949-751X
Elodie BerdanceUniversity of Bordeaux, CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, 33000 Bordeaux, France.
Gertrud Lallinger-KubeDepartment of Genetics, Bldg. NW1, University of Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany.
Annelise SahinUniversity of Bordeaux, CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, 33000 Bordeaux, France.
Denis DacheuxUniversity of Bordeaux, CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, 33000 Bordeaux, France - Institut Polytechnique de Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, 33000, Bordeaux, France.
Nicolas LandreinUniversity of Bordeaux, CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, 33000 Bordeaux, France.
Anne CayrelUniversity of Bordeaux, CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, 33000 Bordeaux, France.
Klaus ErsfeldDepartment of Genetics, Bldg. NW1, University of Bayreuth, Universitätsstraße 30, 95440 Bayreuth, Germany.
Mélanie BonhiversUniversity of Bordeaux, CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, 33000 Bordeaux, France.
Linda KohlUMR 7245 Molécules de Communication et Adaptation des Micro-organismes, Muséum National d'Histoire Naturelle, CNRS, CP52, 61 rue Buffon, 75231 Paris Cedex 05, France.
Derrick R RobinsonUniversity of Bordeaux, CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, 33000 Bordeaux, France.ORCID 0000-0001-5572-4127
Centre National de la Recherche Scientifique · FRUniversity of Bayreuth · DE

Funding

Agence Nationale de la recherche ANR-20-CE91-0003Agence Nationale de la Recherche ANR-09-BLAN-0074Agence Nationale de la Recherche ANR-11-LABX-0024Conseil Régional Aquitaine 20111301014Université de Bordeaux
6 · The paper itself

Abstract

The flagellar pocket (FP) of the pathogen Trypanosoma brucei is an important single copy structure that is formed by the invagination of the pellicular membrane. It is the unique site of endo- and exocytosis and is required for parasite pathogenicity. The FP consists of distinct structural sub-domains with the least explored being the flagellar pocket collar (FPC). TbBILBO1 is the first-described FPC protein of Trypanosoma brucei. It is essential for parasite survival, FP and FPC biogenesis. In this work, we characterize TbKINX1B, a novel TbBILBO1 partner. We demonstrate that TbKINX1B is located on the basal bodies, the microtubule quartet (a set of four microtubules) and the FPC in T. brucei. Down-regulation of TbKINX1B by RNA interference in bloodstream forms is lethal, inducing an overall disturbance in the endomembrane network. In procyclic forms, the RNAi knockdown of TbKINX1B leads to a minor phenotype with a small number of cells displaying epimastigote-like morphologies, with a misplaced kinetoplast. Our results characterize TbKINX1B as the first putative kinesin to be localized both at the basal bodies and the FPC with a potential role in transporting cargo along with the microtubule quartet.

Indexed as

Trypanosoma brucei bruceiFlagellaMicrotubulesProtozoan ProteinsRNA InterferenceProtozoan ProteinsBasal bodiesBILBO1CytoskeletonFlagellar pocket collarKinesinTrypanosome

Identifiers

PMID35262485
PMCPMC8906236
OpenAlexW4220702547

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.