Evidence map›Paper›PMID 35274201›Full record

ReviewMolecular biology reports2022

Research progress of whole-cell-SELEX selection and the application of cell-targeting aptamer.

Yu Duan, Chunyun Zhang, Yuanyuan Wang, Guofu Chen

Abstract readReview
PubMed Publisher
In one paragraph

Review in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
4.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 49 citations in OpenAlex.

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  11. Overview and Prospects of DNA Sequence Visualization.International journal of molecular sciences · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Yu DuanSchool of Marine Science and Technology, Shandong, Harbin Institute of Technology (Weihai), Wenhua West Road, 2#, Weihai, 264209, People's Republic of China.
Chunyun ZhangSchool of Marine Science and Technology, Shandong, Harbin Institute of Technology (Weihai), Wenhua West Road, 2#, Weihai, 264209, People's Republic of China. zhangchunyun@hitwh.edu.cn.
Yuanyuan WangSchool of Marine Science and Technology, Shandong, Harbin Institute of Technology (Weihai), Wenhua West Road, 2#, Weihai, 264209, People's Republic of China.
Guofu ChenSchool of Marine Science and Technology, Shandong, Harbin Institute of Technology (Weihai), Wenhua West Road, 2#, Weihai, 264209, People's Republic of China. chenguofu@hitwh.edu.cn.
Harbin Institute of Technology · CNWeihai Science and Technology Bureau · CN

Funding

HIT Scientific Research Innovation Fund No. HIT.NSRIF.201702National Scientific Foundation of China 41476086National Scientific Foundation of China No. 31600309Shandong Provincial Natural Science Foundation, China ZR2020MD081
6 · The paper itself

Abstract

backgroundAptamers refer to the artificially synthesized nucleic acid sequences (DNA/RNA) that can bind to a wide range of targets with high affinity and specificity, which are generally generated from systematic evolution of ligands by exponential enrichment (SELEX). As a novel method of aptamers screening, whole-cell-SELEX (WC-SELEX) has gained more and more attention in many fields such as biomedicine, analytical chemistry, and molecular diagnostics due to its ability to screen multiple potential aptamers without knowing the detailed structural information of target molecules. METHODS AND

resultsIn recent years, with the deepening of research on application of aptamers, the traditional WC-SELEX cannot meet the practical application because of long experimental period, complicated operation process and low specificity, etc. Therefore, the development of more efficient methods for screening aptamer is always on the road. This paper summarizes the current research status of WC-SELEX for bacteria, parasites and animal cells, and reviews the latest advances of WC-SELEX techniques that are dependent on novel instruments, materials and microelectronics, including fluorescence-activated cell sorting-assisted SELEX, three-dimensional assisted WC-SELEX, and microfluidic chip system-assisted WC-SELEX. In addition, the application of aptamers targeting cells was discussed.

conclusionTaken together, this review is aimed at providing a reference for WC-SELEX selection and application of aptamer targeting cells.

Indexed as

Aptamers, NucleotideSELEX Aptamer TechniqueAnimalsFlow CytometryLigandsAptamers, NucleotideLigandsApplicationAptamerScreening technologyTarget cellsWhole-cell-SELEX

Identifiers

PMID35274201
OpenAlexW4220838223

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.