Evidence map›Paper›PMID 35283781›Full record

ArticleFrontiers in physiology2022

Methods for the Comprehensive

John J Reho, Pablo Nakagawa, Gary C Mouradian, Connie C Grobe, Fatima L Saravia, Colin M L Burnett, Anne E Kwitek, John R Kirby, Jeffrey L Segar, Matthew R Hodges and 2 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
3.1field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 28 citations in OpenAlex.

  1. Titrating cumulative neonatal hyperoxia in mice to model bronchopulmonary dysplasia severities.American journal of respiratory cell and molecular biology · 2026
    Article
  2. β-Arrestin-2 Regulates Sodium Taste Processing.Hypertension (Dallas, Tex. : 1979) · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Maternal low-sodium diet impairs hippocampal neurogenesis and cognition in adult mouse offspring.American journal of physiology. Regulatory, integrative and comparative physiology · 2026
    Article
  7. Article
  8. Article
  9. Angiotensin ATiScience · 2025
    Article
  10. Reutericyclin mitigates risperidone-induced suppression of anaerobic energy expenditure.American journal of physiology. Regulatory, integrative and comparative physiology · 2025
    Article
  11. Review
  12. Article
  13. Early-life sodium restriction programs autonomic dysfunction and salt sensitivity in male C57BL/6J mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2025
    Article
  14. Article
  15. Probiotic and high-fat diet: effects on pain assessment, body composition, and cytokines in male and female adolescent and adult rats.American journal of physiology. Regulatory, integrative and comparative physiology · 2024
    Article
  16. Article
  17. Article
  18. Article
  19. Early-life sodium deprivation programs long-term changes in ingestive behaviors and energy expenditure in C57BL/6J mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2023
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

John J RehoDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, United States.
Pablo NakagawaDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, United States.
Gary C MouradianDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, United States.
Connie C GrobeDepartment of Pediatrics, Medical College of Wisconsin, Milwaukee, WI, United States.
Fatima L SaraviaDepartment of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, United States.
Colin M L BurnettDepartment of Internal Medicine, University of Iowa Hospitals & Clinics, Iowa City, IA, United States.
Anne E KwitekDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, United States.
John R KirbyDepartment of Microbiology and Immunology, Medical College of Wisconsin, Milwaukee, WI, United States.
Jeffrey L SegarDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, United States.
Matthew R HodgesDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, United States.
Curt D SigmundDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, United States.
Justin L GrobeDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, United States.
Medical College of Wisconsin · USUniversity of Iowa Hospitals and Clinics · US

Funding

Clinical and Translational Science AwardUL1TR001436 · NCATS · MEDICAL COLLEGE OF WISCONSIN · PI FREED, JULIE K · 2015 to 2025
$47.5M
Pulmonary Toxicology Facility CoreP30ES005605 · NIEHS · UNIVERSITY OF IOWA · PI Jong Sung Kim · 1990 to 2026
$40.5M
Role of the brain Renin-Angiotensin Sys. in Cardiovas and Metabolic RegulationP01HL084207 · NHLBI · UNIVERSITY OF IOWA · PI GROBE, JUSTIN L · 2007 to 2022
$30.2M
Hybrid Rat Diversity Program - Import of HXB/BXH panelR24OD024617 · OD · MEDICAL COLLEGE OF WISCONSIN · PI DWINELL, MELINDA R · 2018 to 2025
$6.8M
PPARG-dependent Mechanisms Control Endothelial-Smooth Muscle Coordination, Arterial Pressure, Vasomotor Function and Arterial StiffnessR35HL144807 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI SIGMUND, CURT DANIEL · 2019 to 2024
$5.5M
Interaction between leptin and angiotensin in the pathogenesis of obesity-hypertensionR01HL134850 · NHLBI · UNIVERSITY OF IOWA · PI Justin L Grobe · 2017 to 2026
$4.4M
Progressive seizure-induced cardiorespiratory dysfunction in a novel mutant rat model of seizure disorderR01HL122358 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI HODGES, MATTHEW ROBERT · 2015 to 2024
$4.3M
Lysine Acetylation in N. gonorrhoeae Quorum Sensing and Biofilm FormationR01AI108255 · NIAID · UNIVERSITY OF IOWA · PI KIRBY, JOHN R, SCHILLING, BIRGIT · 2014 to 2018
$4.1M
Role of the prorenin receptor in blood pressure and autonomic control through the local activation of the renin angiotensin system in the brainstemK01HL153101 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI NAKAGAWA, PABLO · 2020 to 2024
$562k
Elucidating the role of the gut metagenome in hypertension.F31HL152495 · NHLBI · MEDICAL COLLEGE OF WISCONSIN · PI SARAVIA, FATIMA L · 2020 to 2021
$92k
NCATS NIH HHS UL1 TR001436NHLBI NIH HHS F31 HL152495NHLBI NIH HHS K01 HL153101NHLBI NIH HHS P01 HL084207NHLBI NIH HHS R01 HL122358NHLBI NIH HHS R01 HL134850NHLBI NIH HHS R35 HL144807NIAID NIH HHS R01 AI108255NIEHS NIH HHS P30 ES005605NIH HHS R24 OD024617
6 · The paper itself

Abstract

Cardiovascular disease represents the leading cause of death in the United States, and metabolic diseases such as obesity represent the primary impediment to improving cardiovascular health. Rodent (mouse and rat) models are widely used to model cardiometabolic disease, and as a result, there is increasing interest in the development of accurate and precise methodologies with sufficiently high resolution to dissect mechanisms controlling cardiometabolic physiology in these small organisms. Further, there is great utility in the development of centralized core facilities furnished with high-throughput equipment configurations and staffed with professional content experts to guide investigators and ensure the rigor and reproducibility of experimental endeavors. Here, we outline the array of specialized equipment and approaches that are employed within the Comprehensive Rodent Metabolic Phenotyping Core (CRMPC) and our collaborating laboratories within the Departments of Physiology, Pediatrics, Microbiology & Immunology, and Biomedical Engineering at the Medical College of Wisconsin (MCW), for the detailed mechanistic dissection of cardiometabolic function in mice and rats. We highlight selected methods for the analysis of body composition and fluid compartmentalization, electrolyte accumulation and flux, energy accumulation and flux, physical activity, ingestive behaviors, ventilatory function, blood pressure, heart rate, autonomic function, and assessment and manipulation of the gut microbiota. Further, we include discussion of the advantages and disadvantages of these approaches for their use with rodent models, and considerations for experimental designs using these methods.

Indexed as

blood pressurecardiovascularmetabolicmicrobiomemousephenotypingratventilatory

Identifiers

PMID35283781
PMCPMC8914175
OpenAlexW4214750427

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.