Evidence mapPaperPMID 35288364Full record

ArticleTranslational research : the journal of laboratory and clinical medicine2022

Skeletal muscle MiR-210 expression is associated with mitochondrial function in peripheral artery disease patients.

Ahmed Ismaeel, Emma Fletcher, Dimitrios Miserlis, Marissa Wechsler, Evlampia Papoutsi, Gleb Haynatzki, Robert S Smith, William T Bohannon, Panagiotis Koutakis

Open access · greenAbstract read
In one paragraph

Article in Translational research : the journal of laboratory and clinical medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.2field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 26 citations in OpenAlex.

  1. Review
  2. EV-Encapsulated Mitochondrial miRNAs: Enhancing Cardiomyocyte Bioenergetics.International journal of molecular sciences · 2026
    Review
  3. Article
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  19. Impact of Hypoxia-Induced miR-210 on Pancreatic Cancer.Current issues in molecular biology · 2023
    Review
  20. Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of peripheral artery disease.Translational research : the journal of laboratory and clinical medicine · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Ahmed IsmaeelDepartment of Biology, Baylor University, Waco, Texas.
Emma FletcherDepartment of Biology, Baylor University, Waco, Texas.
Dimitrios MiserlisDepartment of Surgery, University of Texas Health Science Center San Antonio, San Antonio, Texas.
Marissa WechslerDepartment of Biomedical Engineering and Chemical Engineering, University of Texas at San Antonio, San Antonio, Texas.
Evlampia PapoutsiDepartment of Biology, Baylor University, Waco, Texas.
Gleb HaynatzkiDepartment of Biostatistics, University of Nebraska Medical Center, Omaha, Nebraska.
Robert S SmithDepartment of Surgery, Baylor Scott & White Medical Center, Temple, Texas.
William T BohannonDepartment of Surgery, Baylor Scott & White Medical Center, Temple, Texas.
Panagiotis KoutakisDepartment of Biology, Baylor University, Waco, Texas. Electronic address: panagiotis_koutakis@baylor.edu.
Baylor University · USBaylor Scott & White Medical Center - Temple · USThe University of Texas at San Antonio · USThe University of Texas Health Science Center at San Antonio · USUniversity of Nebraska Medical Center · US

Funding

NIA NIH HHS R01 AG064420
6 · The paper itself

Abstract

Previous studies have demonstrated that circulating microRNA (miR)-210 levels are elevated in peripheral artery disease (PAD) patients. MiR-210 is known to be a negative regulator of mitochondrial respiration; however, the relationship between miR-210 and mitochondrial function has yet to be studied in PAD. We aimed to compare skeletal muscle miR-210 expression of PAD patients to non-PAD controls (CON) and to examine the relationship between miR-210 expression and mitochondrial function. Skeletal muscle biopsies from CON (n = 20), intermittent claudication (IC) patients (n = 20), and critical limb ischemia (CLI) patients (n = 20) were analyzed by high-resolution respirometry to measure mitochondrial respiration of permeabilized fibers. Samples were also analyzed for miR-210 expression by real-time PCR. MiR-210 expression was significantly elevated in IC and CLI muscle compared to CON (P = 0.008 and P < 0.001, respectively). Mitochondrial respiration of electron transport chain (ETC) Complexes II (P = 0.001) and IV (P < 0.001) were significantly reduced in IC patients. Further, CLI patients demonstrated significant reductions in respiration during Complexes I (state 2: P = 0.04, state 3: P = 0.003), combined I and II (P < 0.001), II (P < 0.001), and IV (P < 0.001). The expression of the miR-210 targets, cytochrome c oxidase assembly factor heme A: farnesyltransferase (COX10), and iron-sulfur cluster assembly enzyme (ISCU) were down-regulated in PAD muscle. MiR-210 may play a role in the cellular adaptation to hypoxia and may be involved in the metabolic myopathy associated with PAD.

Indexed as

MicroRNAsMitochondriaMuscle, SkeletalPeripheral Arterial DiseaseHumansIntermittent ClaudicationMicroRNAsMIRN210 microRNA, human

Identifiers

PMID35288364
PMCPMC9197925
OpenAlexW4221119374

What Socratic holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.