ArticleProceedings of the National Academy of Sciences of the United States of America2022
Hatching is modulated by microRNA-378a-3p derived from extracellular vesicles secreted by blastocysts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Embryo-derived extracellular vesicles and their potential as biomarkers for embryo quality during IVF: a systematic review.Human reproduction update · 2026Pooled it
- Epididymosome-Supplemented Extender Induces Changes in Morpho-Functional Traits and microRNA Levels of Post-Thaw Sperm and Improves Embryo Developmental Potential.Molecular reproduction and development · 2026Article
- Advances in bovine oocyte in vitro maturation: mechanisms and strategies.Veterinary research communications · 2026Review
- Bovine oviductal and uterine extracellular vesicles enhance blastocyst development in confined individual embryo culture.Journal of assisted reproduction and genetics · 2026Article
- Extracellular vesicles in bovine reproduction: their journey from gametogenesis to pregnancy.Frontiers in cell and developmental biology · 2026Review
- Integrated molecular analysis of granulosa cells and follicular fluid extracellular vesicles reveals predictive biomarkers of oocyte maturation in the southern white rhinoceros.Frontiers in cell and developmental biology · 2026Article
- Extracellular Vesicles in Implantation: Cross-Talk Between the Embryo and Endometrium.Advances in anatomy, embryology, and cell biology · 2026Review
- Purification and Characterization of Extracellular Vesicles from Embryo-Conditioned Medium.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Reproductive fluids, commercial media, and organoids: bridging the gap in IVF culture systems.Human reproduction open · 2026Review
- [The role of extracellular vesicles in early embryo development and their application in assisted reproductive technologies].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2025Review
- The Molecular Dynamics of Extracellular Vesicles and their Protein Corona in the Secretomes of Stem Cells.Stem cell reviews and reports · 2025Review
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- Follicular-fluid extracellular vesicles support energy metabolism of bovine oocytes, improving blastocyst development and quality†.Biology of reproduction · 2025Article
- Theranostic potential of extracellular vesicles in reproductive tracts: implications for recurrent implantation failure.Molecular biology reports · 2025Review
- Noninvasive Biomarkers of Human Embryo Developmental Potential.International journal of molecular sciences · 2025Review
- Extracellular vesicles: key mediators inFrontiers in veterinary science · 2025Review
- Biogenesis of Extracellular Vesicles (EVs) and the Potential Use of Embryo-Derived EVs in Medically Assisted Reproduction.International journal of molecular sciences · 2024Review
- An atlas of small non-coding RNAs in human preimplantation development.Nature communications · 2024Article
- Altered microRNA composition in the uterine lumen fluid in cattle (Bos taurus) pregnancies initiated by artificial insemination or transfer of an in vitro produced embryo.Journal of animal science and biotechnology · 2024Article
Corrections and comments
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Authors and funding
16 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SignificanceHatching from the zona pellucida is a prerequisite for embryo implantation and is less likely to occur in vitro for reasons unknown. Extracellular vesicles (EVs) are secreted by the embryo into the culture medium. Yet the role that embryonic EVs and their cargo microRNAs (miRNAs) play in blastocyst hatching has not been elucidated, partially due to the difficulties of isolating them from low amounts of culture medium. Here, we optimized EV-miRNA isolation from medium conditioned by individually cultured bovine embryos and subsequently showed that miR-378a-3p, which was up-regulated in EVs secreted by blastocysts, plays a crucial role in promoting blastocyst hatching. This demonstrates the regulatory effect of miR-378-3p on hatching, which is an established embryo quality parameter linked with implantation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.