ArticleScientific reports2022
Identifying optimal reference genes for gene expression studies in Eurasian spruce bark beetle, Ips typographus (Coleoptera: Curculionidae: Scolytinae).
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 18 citations in OpenAlex.
- Screening of ester-forming and hydrolyzing enzymes linked to pheromone production in Ips typographus (Linnaeus, 1758).BMC genomics · 2026Article
- Detoxification of conifer antimicrobial defenses promotes entomopathogenic fungus infection of bark beetles.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Stability and suitability of housekeeping genes in phlebotomine sand flies.Scientific reports · 2024Article
- Insights into the Detoxification of Spruce Monoterpenes by the Eurasian Spruce Bark Beetle.International journal of molecular sciences · 2024Article
- Article
- Evaluation of reference genes for quantitative expression analysis inFrontiers in physiology · 2024Article
- Screening of reference genes for microRNA analysis in the study of solider caste differentiation of Formosan subterranean termite Coptotermes formosanus Shiraki.Scientific reports · 2023Article
- Nanoparticle-Shielded dsRNA Delivery for Enhancing RNAi Efficiency in Cotton Spotted BollwormInternational journal of molecular sciences · 2023Article
- Comparative gut proteomics study revealing adaptive physiology of Eurasian spruce bark beetle,Frontiers in plant science · 2023Article
- Reference Genes for Expression Analysis Using RT-qPCR inInsects · 2022Article
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5 authors at 1 institution in 1 country.
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Abstract
Eurasian spruce bark beetle (Ips typographus [L.]) causes substantial damage to spruce forests worldwide. Undoubtedly, more aggressive measures are necessary to restrict the enduring loss. Finishing genome sequencing is a landmark achievement for deploying molecular techniques (i.e., RNA interference) to manage this pest. Gene expression studies assist in understanding insect physiology and deployment of molecular approaches for pest management. RT-qPCR is a valuable technique for such studies. However, accuracy and reliability depend on suitable reference genes. With the genome sequence available and the growing requirement of molecular tools for aggressive forest pest management, it is crucial to find suitable reference genes in Ips typographus under different experimental conditions. Hence, we evaluated the stability of twelve candidate reference genes under diverse experimental conditions such as biotic (developmental, sex and tissues) and abiotic factors (i.e., temperature and juvenile hormone treatment) to identify the reference genes. Our results revealed that ribosomal protein 3a (RPS3-a) was the best reference gene across all the experimental conditions, with minor exceptions. However, the stability of the reference gene can differ based on experiments. Nevertheless, present study provides a comprehensive list of reference genes under different experimental conditions for Ips typographus and contributes to "future genomic and functional genomic research".
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