Evidence map›Paper›PMID 35313741›Full record

ArticleCartilage

Stiffness- and Bioactive Factor-Mediated Protection of Self-Assembled Cartilage against Macrophage Challenge in a Novel Co-Culture System.

Ryan P Donahue, Jarrett M Link, Vijaykumar S Meli, Jerry C Hu, Wendy F Liu, Kyriacos A Athanasiou

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cartilage. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. Astaxanthin mediated repair of tBHP-Induced cellular injury in chondrocytes.Redox report : communications in free radical research · 2024
    Article
  4. Review
  5. Review
  6. Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Ryan P DonahueDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.ORCID 0000-0003-0542-1034
Jarrett M LinkDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.ORCID 0000-0001-9108-1456
Vijaykumar S MeliDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
Jerry C HuDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
Wendy F LiuDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
Kyriacos A AthanasiouDepartment of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.ORCID 0000-0001-5387-8405
University of California, Irvine · US

Funding

TMJ Disc RegenerationR01DE015038 · NIDCR · UNIVERSITY OF CALIFORNIA-IRVINE · PI ATHANASIOU, KYRIACOS A · 2004 to 2022
$6.1M
Biophysical regulation of macrophage functionR01AI151301 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI LIU, WENDY · 2020 to 2024
$2.9M
Self-assembling process in tissue engineering of articular cartilageR01AR067821 · NIAMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI ATHANASIOU, KYRIACOS A · 2015 to 2019
$1.9M
NIAID NIH HHS R01 AI151301NIAMS NIH HHS R01 AR067821NIDCR NIH HHS R01 DE015038
6 · The paper itself

Abstract

objectiveTissue-engineered cartilage implants must withstand the potential inflammatory and joint loading environment for successful long-term repair of defects. The work's objectives were to develop a novel, direct cartilage-macrophage co-culture system and to characterize interactions between self-assembled neocartilage and differentially stimulated macrophages.

designIn study 1, it was hypothesized that the proinflammatory response of macrophages would intensify with increasing construct stiffness; it was expected that the neocartilage would display a decrease in mechanical properties after co-culture. In study 2, it was hypothesized that bioactive factors would protect neocartilage properties during macrophage co-culture. Also, it was hypothesized that interleukin 10 (IL-10)-stimulated macrophages would improve neocartilage mechanical properties compared to lipopolysaccharide (LPS)-stimulated macrophages.

resultsAs hypothesized, stiffer neocartilage elicited a heightened proinflammatory macrophage response, increasing tumor necrosis factor alpha (TNF-α) secretion by 5.47 times when LPS-stimulated compared to construct-only controls. Interestingly, this response did not adversely affect construct properties for the stiffest neocartilage but did correspond to a significant decrease in aggregate modulus for soft and medium stiffness constructs. In addition, bioactive factor-treated constructs were protected from macrophage challenge compared to chondrogenic medium-treated constructs, but IL-10 did not improve neocartilage properties, although stiff constructs appeared to bolster the anti-inflammatory nature of IL-10-stimulated macrophages. However, co-culture of bioactive factor-treated constructs with LPS-treated macrophages reduced TNF-α secretion by over 4 times compared to macrophage-only controls.

conclusionsIn conclusion, neocartilage stiffness can mediate macrophage behavior, but stiffness and bioactive factors prevent macrophage-induced degradation. Ultimately, this co-culture system could be utilized for additional studies to develop the burgeoning field of cartilage mechano-immunology.

Indexed as

Cartilage, ArticularChondrocytesCoculture TechniquesInterleukin-10LipopolysaccharidesMacrophagesTumor Necrosis Factor-alphaInterleukin-10LipopolysaccharidesTumor Necrosis Factor-alphacartilageimmunologymacrophagetissue engineering

Identifiers

PMID35313741
PMCPMC9137312
OpenAlexW4220817683

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.