Evidence map›Paper›PMID 35314699›Full record

ArticleNature communications2022

Transposon insertional mutagenesis of diverse yeast strains suggests coordinated gene essentiality polymorphisms.

Piaopiao Chen, Agnès H Michel, Jianzhi Zhang

Open access · goldAbstract read
In one paragraph

Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Piaopiao ChenDepartment of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, 48109, USA.ORCID http://orcid.org/0000-0003-4659-816X
Agnès H MichelDepartment of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK.
Jianzhi ZhangDepartment of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI, 48109, USA. jianzhi@umich.edu.ORCID http://orcid.org/0000-0001-6141-1290
University of Michigan · USUniversity of Oxford · GB

Funding

Genomic and Systemic Approaches to Evolutionary MechanismsR35GM139484 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI JIANZHI ZHANG · 2021 to 2026
$3.2M
NIGMS NIH HHS R35 GM139484
6 · The paper itself

Abstract

Due to epistasis, the same mutation can have drastically different phenotypic consequences in different individuals. This phenomenon is pertinent to precision medicine as well as antimicrobial drug development, but its general characteristics are largely unknown. We approach this question by genome-wide assessment of gene essentiality polymorphism in 16 Saccharomyces cerevisiae strains using transposon insertional mutagenesis. Essentiality polymorphism is observed for 9.8% of genes, most of which have had repeated essentiality switches in evolution. Genes exhibiting essentiality polymorphism lean toward having intermediate numbers of genetic and protein interactions. Gene essentiality changes tend to occur concordantly among components of the same protein complex or metabolic pathway and among a group of over 100 mitochondrial proteins, revealing molecular machines or functional modules as units of gene essentiality variation. Most essential genes tolerate transposon insertions consistently among strains in one or more coding segments, delineating nonessential regions within essential genes.

Indexed as

DNA Transposable ElementsSaccharomyces cerevisiaeGenes, EssentialHumansMetabolic Networks and PathwaysMutagenesisMutagenesis, InsertionalDNA Transposable Elements

Identifiers

PMID35314699
PMCPMC8938418
OpenAlexW4220995072

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.