ArticleJournal of functional biomaterials2022
Engineering Nanopatterned Structures to Orchestrate Macrophage Phenotype by Cell Shape.
Article in Journal of functional biomaterials, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Enhancing skin-implant integration in lower-limb transcutaneous prostheses: From interface biology to bioactive, antimicrobial and cell-based strategies.Journal of orthopaedic translation · 2026Review
- Morpho-functional plasticity of tumour-associated macrophages: linking cell shape, ultrastructure, and immune regulation in cancer.Clinical and experimental medicine · 2026Review
- Multiscale Interface Engineering for Orthopedic and Dental Implants: A Review.Journal of functional biomaterials · 2026Review
- Diameter-dependent effects of nanopillar arrays on PEEK bioactivity and soft tissue integration.Journal of nanobiotechnology · 2026Article
- Light-Responsive Surface Topographies Modulate Macrophage Immune Responses Through Dynamic Mechanical Cues.Macromolecular bioscience · 2026Article
- Poly-l-lactic acid electrospun membrane with specific morphology promotes bone regeneration through macrophage reprogramming.Journal of nanobiotechnology · 2026Article
- Picosecond laser-engineered osteon-inspired concentric micropatterns on titanium implants regulate cellular behaviour to facilitate osseointegration.Materials today. Bio · 2026Article
- Peptide-Functionalized Nanopillared Surfaces with Tunable Antimicrobial and Immunomodulatory Properties.ACS omega · 2025Article
- TPMS-Gyroid Scaffold-Mediated Up-Regulation of ITGB1 for Enhanced Cell Adhesion and Immune-Modulatory Osteogenesis.Advanced healthcare materials · 2025Article
- Chemoresistant tumor cell secretome potentiates immune suppression in triple negative breast cancer.Breast cancer research : BCR · 2025Article
- Multifunctional micro/nano-textured titanium with bactericidal, osteogenic, angiogenic and anti-inflammatory properties: Insights fromMaterials today. Bio · 2025Article
- Dynamic hierarchical ligand anisotropy for competing macrophage regulationBioactive materials · 2025Article
- Feature Papers in Bone Biomaterials.Journal of functional biomaterials · 2024Article
- Electrospun Fiber Surface Roughness Modulates Human Monocyte-Derived Macrophage Phenotype.bioRxiv : the preprint server for biology · 2024Article
- Unveiling the Potential of Migrasomes: A Machine-Learning-Driven Signature for Diagnosing Acute Myocardial Infarction.Biomedicines · 2024Article
- The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in Osteoarthritis.Medical science monitor : international medical journal of experimental and clinical research · 2024Article
- Copper metabolism in osteoarthritis and its relation to oxidative stress and ferroptosis in chondrocytes.Frontiers in molecular biosciences · 2024Review
- Effect of Porous Substrate Topographies on Cell Dynamics: A Computational Study.ACS biomaterials science & engineering · 2023Article
- The Effect of Titanium Surface Topography on Adherent Macrophage Integrin and Cytokine Expression.Journal of functional biomaterials · 2023Article
- The 3D-Printed Ordered Bredigite Scaffold Promotes Pro-Healing of Critical-Sized Bone Defects by Regulating Macrophage Polarization.International journal of nanomedicine · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Physical features on the biomaterial surface are known to affect macrophage cell shape and phenotype, providing opportunities for the design of novel "immune-instructive" topographies to modulate foreign body response. The work presented here employed nanopatterned polydimethylsiloxane substrates with well-characterized nanopillars and nanopits to assess RAW264.7 macrophage response to feature size. Macrophages responded to the small nanopillars (SNPLs) substrates (450 nm in diameter with average 300 nm edge-edge spacing), resulting in larger and well-spread cell morphology. Increasing interpillar distance to 800 nm in the large nanopillars (LNPLs) led to macrophages exhibiting morphologies similar to being cultured on the flat control. Macrophages responded to the nanopits (NPTs with 150 nm deep and average 800 nm edge-edge spacing) by a significant increase in cell elongation. Elongation and well-spread cell shape led to expression of anti-inflammatory/pro-healing (M2) phenotypic markers and downregulated expression of inflammatory cytokines. SNPLs and NPTs with high availability of integrin binding region of fibronectin facilitated integrin β1 expression and thus stored focal adhesion formation. Increased integrin β1 expression in macrophages on the SNPLs and NTPs was required for activation of the PI3K/Akt pathway, which promoted macrophage cell spreading and negatively regulated NF-κB activation as evidenced by similar globular cell shape and higher level of NF-κB expression after PI3K blockade. These observations suggested that alterations in macrophage cell shape from surface nanotopographies may provide vital cues to orchestrate macrophage phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.