ArticleMetabolites2022
Fast and Sensitive Quantification of AccQ-Tag Derivatized Amino Acids and Biogenic Amines by UHPLC-UV Analysis from Complex Biological Samples.
Article in Metabolites, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- A Rapid and Validated Reversed-Phase High-Performance Liquid Chromatographic Method with Pre-Column Derivatization and Fluorescence Detection for Quantification of Twenty Amino Acids in Total Parenteral Nutrition Solutions.Journal of chromatographic science · 2026Article
- Leaf protein concentrate as a nutrient-dense protein source from vetch and triticale mixture: A comparative analysis of fermentation techniques.Food chemistry: X · 2025Article
- Profiling HydrophilicPlants (Basel, Switzerland) · 2025Article
- Comprehensive Profiling of Free Proteinogenic and Non-Proteinogenic Amino Acids in Common Legumes Using LC-QToF: Targeted and Non-Targeted Approaches.Foods (Basel, Switzerland) · 2025Article
- Enhanced Oligopeptide and Free Tryptophan Release from Chickpea and Lentil Proteins: A Comparative Study of Enzymatic Modification with Bromelain, Ficin, and Papain.Plants (Basel, Switzerland) · 2024Article
- Urinary metabolic characterization of advanced tuberculous meningitis cases in a South African paediatric population.Frontiers in molecular biosciences · 2024Article
- Comparative Analysis of Amino Acid and Biogenic Amine Compositions of Fermented Grape Beverages.Metabolites · 2023Article
- Immunomodulatory and Antioxidant Properties of a Novel Potential ProbioticMicroorganisms · 2023Article
- Human abdominal subcutaneous-derived active beige adipocytes carryingFrontiers in cell and developmental biology · 2023Article
- Serum amino acids quantification by plasmonic colloidosome-coupled MALDI-TOF MS for triple-negative breast cancer diagnosis.Materials today. Bio · 2022Article
- Comparison of Wet Fractionation Methods for Processing Broccoli Agricultural Wastes and Evaluation of the Nutri-Chemical Values of Obtained Products.Foods (Basel, Switzerland) · 2022Article
- Metabolomic Analysis of Serum and Tear Samples from Patients with Obesity and Type 2 Diabetes Mellitus.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Metabolomic analysis of different body fluids bears high importance in medical sciences. Our aim was to develop and validate a fast UHPLC-UV method for the analysis of 33 amino acids and biogenic amines from complex biological samples. AccQ-Tag derivatization was conducted on target molecules and the derivatized targets were analyzed by UHPLC-UV. The detection of the analytes was carried out with UV analysis and by Selected Reaction Monitoring (SRM)-based targeted mass spectrometry. The method was validated according to the FDA guidelines. Serum and non-stimulated tear samples were collected from five healthy individuals and the samples were analyzed by the method. The method was successfully validated with appropriate accuracy and precision for all 33 biomolecules. A total of 29 analytes were detected in serum samples and 26 of them were quantified. In the tears, 30 amino acids and biogenic amines were identified and 20 of them were quantified. The developed and validated UHPLC-UV method enables the fast and precise analysis of amino acids and biogenic amines from complex biological samples.
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