Evidence mapPaperPMID 35345489Full record

ArticleFrontiers in cardiovascular medicine2022

Exosome-Derived From Sepsis Patients' Blood Promoted Pyroptosis of Cardiomyocytes by Regulating miR-885-5p/HMBOX1.

Guo-Wei Tu, Jie-Fei Ma, Jia-Kun Li, Ying Su, Jing-Chao Luo, Guang-Wei Hao, Ming-Hao Luo, Yi-Rui Cao, Yi Zhang, Zhe Luo

Open access · goldAbstract read
In one paragraph

Article in Frontiers in cardiovascular medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 7 institutions in 1 country.

Guo-Wei TuDepartment of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Jie-Fei MaDepartment of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Jia-Kun LiDepartment of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Ying SuDepartment of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Jing-Chao LuoDepartment of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Guang-Wei HaoDepartment of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Ming-Hao LuoShanghai Medical College, Fudan University, Shanghai, China.
Yi-Rui CaoShanghai Key Laboratory of Organ Transplantation, Shanghai, China.
Yi ZhangShanghai Key Laboratory of Organ Transplantation, Shanghai, China.
Zhe LuoDepartment of Critical Care Medicine, Zhongshan Hospital, Fudan University, Shanghai, China.
Fudan University · CNSun Yat-sen University · CNThe First Affiliated Hospital, Sun Yat-sen University · CNShanghai Institute of Organic Chemistry · CNShanghai Medical College of Fudan University · CNZhongshan Hospital · CNZhongshan Hospital of Xiamen University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Septic myocardial depression has been associated with increased morbidity and mortality. miR-885-5p has been shown to regulate cell growth, senescence, and/or apoptosis. Published studies demonstrated that Homeobox-containing protein 1 (HMBOX1) inhibits inflammatory response, regulates cell autophagy, and apoptosis. However, the role of miR-885-5p/HMBOX1 in sepsis and septic myocardial depression and the underlying mechanism is not fully understood. Materials and Methods: Exosomes (exos) derived from sepsis patients (sepsis-exos) were isolated using ultracentrifugation. Rats were subjected to cecal ligation and puncture surgery and treated with sepsis-exos. HMBOX1 was knocked down or overexpressed in AC16 cells using lentiviral plasmids carrying short interfering RNAs targeting human HMBOX1 or carrying HMBOX1 cDNA. Cell pyroptosis was measured by flow cytometry. The secretion of IL-1β and IL-18 was examined by ELISA kits. Quantitative polymerase chain reaction (PCR) or western blot was used for gene expression. Results: Sepsis-exos increased the level of miR-885-5p, decreased HMBOX1, elevated IL-1β and IL-18, and promoted pyroptosis in AC16 cells. Septic rats treated with sepsis-exos increased the serum inflammatory cytokines is associated with increased pyroptosis-related proteins of hearts. MiR-885-5p bound to the three prime untranslated regions of HMBOX1 to negatively regulate its expression. Overexpressing HMBOX1 reversed miR-885-5p-induced elevation of inflammatory cytokines and upregulation of NLRP3, caspase-1, and GSDMD-N in AC16 cells. The mechanistic study indicated that the effect of HMBOX1 was NF-κB dependent. Conclusion: Sepsis-exos promoted the pyroptosis of AC16 cells through miR-885-5p

Indexed as

cardiomyocytesexosomesmicroRNApyroptosissepsis

Identifiers

PMID35345489
PMCPMC8957255
OpenAlexW4220705618

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.