Evidence map›Paper›PMID 35406647›Full record

ArticleCells2022

Lichen Secondary Metabolites Inhibit the Wnt/β-Catenin Pathway in Glioblastoma Cells and Improve the Anticancer Effects of Temozolomide.

Aleksandra Majchrzak-Celińska, Robert Kleszcz, Elżbieta Studzińska-Sroka, Agnieszka Łukaszyk, Anna Szoszkiewicz, Ewelina Stelcer, Karol Jopek, Marcin Rucinski, Judyta Cielecka-Piontek, Violetta Krajka-Kuźniak

Open access · goldAbstract read
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
11.6field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 31 citations in OpenAlex.

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  7. Pleiotropic Potential ofMolecules (Basel, Switzerland) · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Aleksandra Majchrzak-CelińskaDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Święcicki 4 Str., 60-781 Poznań, Poland.ORCID 0000-0002-2872-8875
Robert KleszczDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Święcicki 4 Str., 60-781 Poznań, Poland.ORCID 0000-0002-6607-7436
Elżbieta Studzińska-SrokaDepartment of Pharmacognosy, Poznan University of Medical Sciences, Rokietnicka 3 Str., 60-806 Poznań, Poland.ORCID 0000-0002-0462-1766
Agnieszka ŁukaszykDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Święcicki 4 Str., 60-781 Poznań, Poland.
Anna SzoszkiewiczDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Święcicki 4 Str., 60-781 Poznań, Poland.
Ewelina StelcerDepartment of Histology and Embryology, Poznan University of Medical Sciences, Święcicki 6 Str., 60-781 Poznań, Poland.
Karol JopekDepartment of Histology and Embryology, Poznan University of Medical Sciences, Święcicki 6 Str., 60-781 Poznań, Poland.ORCID 0000-0002-7399-0303
Marcin RucinskiDepartment of Histology and Embryology, Poznan University of Medical Sciences, Święcicki 6 Str., 60-781 Poznań, Poland.ORCID 0000-0002-2525-5777
Judyta Cielecka-PiontekDepartment of Pharmacognosy, Poznan University of Medical Sciences, Rokietnicka 3 Str., 60-806 Poznań, Poland.ORCID 0000-0003-0891-5419
Violetta Krajka-KuźniakDepartment of Pharmaceutical Biochemistry, Poznan University of Medical Sciences, Święcicki 4 Str., 60-781 Poznań, Poland.ORCID 0000-0001-7275-0298
Poznan University of Medical Sciences · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lichens are a source of secondary metabolites with significant pharmacological potential. Data regarding their possible application in glioblastoma (GBM) treatment are, however, scarce. The study aimed at analyzing the mechanism of action of six lichen secondary metabolites: atranorin, caperatic acid, physodic acid, squamatic acid, salazinic acid, and lecanoric acid using two- and three-dimensional GBM cell line models. The parallel artificial membrane permeation assay was used to predict the blood-brain barrier penetration ability of the tested compounds. Their cytotoxicity was analyzed using the MTT test on A-172, T98G, and U-138 MG cells. Flow cytometry was applied to the analysis of oxidative stress, cell cycle distribution, and apoptosis, whereas qPCR and microarrays detected the induced transcriptomic changes. Our data confirm the ability of lichen secondary metabolites to cross the blood-brain barrier and exert cytotoxicity against GBM cells. Moreover, the compounds generated oxidative stress, interfered with the cell cycle, and induced apoptosis in T98G cells. They also inhibited the Wnt/β-catenin pathway, and this effect was even stronger in case of a co-treatment with temozolomide. Transcriptomic changes in cancer related genes induced by caperatic acid and temozolomide were the most pronounced. Lichen secondary metabolites, caperatic acid in particular, should be further analyzed as potential anti-GBM agents.

Indexed as

GlioblastomaLichensTemozolomideWnt Signaling Pathwaybeta CateninCell Line, TumorHumansbeta CateninTemozolomideblood-brain barrier permeabilitycaperatic acidglioblastomalichen secondary metabolitesmicroarraysphysodic acidtemozolomideWnt/β-catenin pathway

Identifiers

PMID35406647
PMCPMC8997913
OpenAlexW4220872334

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.