Evidence map›Paper›PMID 35409428›Full record

ArticleInternational journal of molecular sciences2022

Circ003429 Regulates Unsaturated Fatty Acid Synthesis in the Dairy Goat Mammary Gland by Interacting with miR-199a-3p, Targeting the

Peixin Jiao, Meimei Zhang, Ziwei Wang, Gege Liang, Xiaolai Xie, Yonggen Zhang, Zhi Chen, Qianming Jiang, Juan J Loor

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.9field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Review
  6. Article
  7. Article
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Peixin JiaoCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.ORCID 0000-0001-9416-7503
Meimei ZhangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Ziwei WangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Gege LiangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Xiaolai XieCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Yonggen ZhangCollege of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.
Zhi ChenJiangsu Key Laboratory of Animal Genetic Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.ORCID 0000-0003-3924-1891
Qianming JiangMammalian Nutrition Physiology Genomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA.
Juan J LoorMammalian Nutrition Physiology Genomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, IL 61801, USA.ORCID 0000-0003-1586-4365
Northeast Agricultural University · CNUniversity of Illinois Urbana-Champaign · USYangzhou University · CN

Funding

National Natural Science Foundation of China 31902182
6 · The paper itself

Abstract

Fatty acid composition is a key factor affecting the flavor and quality of goat milk. CircRNAs are now recognized as important regulators of transcription, and they play an important role in the control of fatty acid synthesis. Thus, understanding the regulatory mechanisms controlling this process in ruminant mammary glands is of great significance. In the present study, mammary tissue from dairy goats during early lactation and the dry period (nonlactating) were collected and used for high-throughput sequencing. Compared to levels during the dry period, the expression level of circ003429 during early lactation was lower (12.68-fold downregulated). In isolated goat mammary epithelial cells, circ003429 inhibited the synthesis of triglycerides (TAG) and decreased the content of unsaturated fatty acids (C16:1, C18:1, and C18:2), indicating that this circRNA plays an important role in regulating lipid synthesis. A binding site for miR-199a-3p in the circ003429 sequence was detected, and a dual-luciferase reporter system revealed that circ003429 targets miR-199a-3p. Overexpression of circ003429 (pcDNA-circ003429) downregulated the abundance of miR-199a-3p. In contrast, overexpression of miR-199a-3p increased TAG content and decreased mRNA abundance of Yes-associated protein 1 (

Indexed as

Mammary Glands, AnimalMicroRNAsAnimalsEpithelial CellsFatty AcidsFatty Acids, UnsaturatedFemaleGoatsRNA, CircularRNA, MessengerFatty AcidsFatty Acids, UnsaturatedMicroRNAsRNA, CircularRNA, Messengercirc003429fatty acid synthesisgoat mammary epithelial cellsmiR-199a-3pYAP1

Identifiers

PMID35409428
PMCPMC8999533
OpenAlexW4223556278

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.