ArticleDevelopmental cell2022
SALSA, a genetically encoded biosensor for spatiotemporal quantification of Notch signal transduction in vivo.
Article in Developmental cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Article
- Regulation of hlh-2 transcription during specification of the anchor cell of the C. elegans hermaphrodite gonad.G3 (Bethesda, Md.) · 2026Article
- Niche-associated Type IV collagen promotes GLP-1/Notch receptor activation in the C. elegans germline.Nature communications · 2025Article
- Cell lineage-resolved embryonic morphological map reveals signaling associated with cell fate and size asymmetry.Nature communications · 2025Article
- Loss of the distal-proximal GLP-1/Notch activation gradient in the agingmicroPublication biology · 2025Article
- Enabling neighbour labelling: using synthetic biology to explore how cells influence their neighbours.Development (Cambridge, England) · 2024Review
- Mutation of GLP-1/Notch RAM domain results in a strong Glp-1 phenotype.microPublication biology · 2024Article
- Article
- Visualizing and quantifying molecular and cellular processes in Caenorhabditis elegans using light microscopy.Genetics · 2022Review
- A new toolkit to visualize and perturb endogenous LIN-12/Notch signaling inmicroPublication biology · 2022Article
- Redundant mechanisms regulating the proliferation vs. differentiation balance in theFrontiers in cell and developmental biology · 2022Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Notch-mediated lateral specification is a fundamental mechanism to resolve stochastic cell fate choices by amplifying initial differences between equivalent cells. To study how stochastic events impact Notch activity, we developed a biosensor, SALSA (sensor able to detect lateral signaling activity), consisting of an amplifying "switch"-Notch tagged with TEV protease-and a "reporter"-GFP fused to a nuclearly localized red fluorescent protein, separated by a TEVp cut site. When ligand activates Notch, TEVp enters the nucleus and releases GFP from its nuclear tether, allowing Notch activation to be quantified based on the changes in GFP subcellular localization. We show that SALSA accurately reports Notch activity in different signaling paradigms in Caenorhabditis elegans and use time-lapse imaging to test hypotheses about how stochastic elements ensure a reproducible and robust outcome in a canonical lin-12/Notch-mediated lateral signaling paradigm. SALSA should be generalizable to other experimental systems and be adaptable to increase options for bespoke "SynNotch" applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.