ArticleNature microbiology2022
Anopheline mosquitoes are protected against parasite infection by tryptophan catabolism in gut microbiota.
Article in Nature microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
- Discovery of functional compounds from dimorphic edibleSynthetic and systems biotechnology · 2026Article
- Cross-species TNF-α signaling in mosquitoes promotes malaria transmission.EMBO reports · 2026Article
- Immune-mediated microbial interference governsiScience · 2026Article
- Host macrophages/monocytes promote malaria transmission by modulating mosquito microbiota via SR-A-mediated phagocytosis.Nature communications · 2026Article
- Article
- Reactive oxygen species-producing genes regulate mosquito midgut bacteria colonization, transcriptomic changes and cell repair.Communications biology · 2026Article
- Disruption of Gut Microbiota-Mediated De Novo NADAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Bacterial community composition and diversity associated with developmental stages of Anopheles subpictus.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Avian malaria and the overlooked metabolic pathways underlying mosquitoRoyal Society open science · 2025Article
- Microbiome diversity in mosquitoes and sand flies: implications for vector competence.Parasites & vectors · 2025Review
- Seasonal Plasticity in Tryptophan Metabolism Provides New Insights Into Physiological Adaptation in Snake Hibernation.Ecology and evolution · 2025Article
- Emerging challenges for mosquito-borne disease control and the promise of symbiont-based transmission-blocking strategies.PLoS pathogens · 2025Review
- Host complement C3 promotes malaria transmission by killing symbiotic bacteria in the mosquito midgut.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Gut Microbial Tryptophan Metabolism Is Involved in Post-Cardiac Arrest Brain Injury via Pyroptosis Modulation.CNS neuroscience & therapeutics · 2025Article
- Cooperative microbial metabolism enhances tryptophan-mediated insecticide detoxification in the fall armyworm.The ISME journal · 2025Article
- Targeting revascularization via tryptophan-indole-NETs axis: the synergistic power of acupuncture and rt-PA thrombolysis in ischemic stroke.Frontiers in neurology · 2025Article
- Cell wall components of gut commensal bacteria stimulate peritrophic matrix formation in malaria vector mosquitoes through activation of the IMD pathway.PLoS biology · 2025Article
- Host 5-HT affects Plasmodium transmission in mosquitoes via modulating mosquito mitochondrial homeostasis.PLoS pathogens · 2024Article
- Relationship between deltamethrin resistance and gut symbiotic bacteria of Aedes albopictus by 16S rDNA sequencing.Parasites & vectors · 2024Article
- Exploring the Larvicidal and Adulticidal Activity againstMolecules (Basel, Switzerland) · 2024Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The mosquito microbiota can influence host physiology and vector competence, but a detailed understanding of these processes is lacking. Here we found that the gut microbiota of Anopheles stephensi, a competent malaria vector, is involved in tryptophan metabolism and is responsible for the catabolism of the peritrophic matrix impairing tryptophan metabolites. Antibiotic elimination of the microbiota led to the accumulation of tryptophan and its metabolites-kynurenine, 3-hydroxykynurenine (3-HK) and xanthurenic acid. Of these metabolites, 3-HK impaired the structure of the peritrophic matrix and promoted Plasmodium berghei infection. Among the major gut microbiota members in A. stephensi, Pseudomonas alcaligenes catabolized 3-HK as revealed by whole-genome sequencing and LC-MS metabolic analysis. The genome of P. alcaligenes encodes kynureninase (KynU) that is responsible for the conversion of 3-HK to 3-hydroxyanthranilic acid. Mutation of KynU resulted in a P. alcaligenes strain that was unable to metabolize 3-HK and unable to protect the peritrophic matrix. Colonization of A. stephensi with KynU-mutated P. alcaligenes failed to protect mosquitoes against parasite infection as compared with mosquitoes colonized with wild-type P. alcaligenes. In summary, this study identifies an unexpected function of mosquito gut microbiota in controlling mosquito tryptophan metabolism, with important implications for vector competence.
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