Evidence map›Paper›PMID 35455937›Full record

ArticleCells2022

Endo- and Exometabolome Crosstalk in Mesenchymal Stem Cells Undergoing Osteogenic Differentiation.

Daniela S C Bispo, Lenka Michálková, Marlene Correia, Catarina S H Jesus, Iola F Duarte, Brian J Goodfellow, Mariana B Oliveira, João F Mano, Ana M Gil

Open access · goldAbstract read
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. MSTNCells · 2026
    Article
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  7. PdJournal of medicinal chemistry · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Daniela S C BispoDepartment of Chemistry, University of Aveiro, CICECO-Aveiro Institute of Materials (CICECO/UA), 3810-193 Aveiro, Portugal.ORCID 0000-0002-5813-5421
Lenka MichálkováDepartment of Analytical Chemistry, University of Chemistry and Technology, 166 28 Prague, Czech Republic.ORCID 0000-0002-8119-004X
Marlene CorreiaDepartment of Chemistry, University of Aveiro, CICECO-Aveiro Institute of Materials (CICECO/UA), 3810-193 Aveiro, Portugal.ORCID 0000-0002-2026-9088
Catarina S H JesusDepartment of Chemistry, University of Aveiro, CICECO-Aveiro Institute of Materials (CICECO/UA), 3810-193 Aveiro, Portugal.ORCID 0000-0003-1812-1663
Iola F DuarteDepartment of Chemistry, University of Aveiro, CICECO-Aveiro Institute of Materials (CICECO/UA), 3810-193 Aveiro, Portugal.ORCID 0000-0003-4289-9256
Brian J GoodfellowDepartment of Chemistry, University of Aveiro, CICECO-Aveiro Institute of Materials (CICECO/UA), 3810-193 Aveiro, Portugal.
Mariana B OliveiraDepartment of Chemistry, University of Aveiro, CICECO-Aveiro Institute of Materials (CICECO/UA), 3810-193 Aveiro, Portugal.
João F ManoDepartment of Chemistry, University of Aveiro, CICECO-Aveiro Institute of Materials (CICECO/UA), 3810-193 Aveiro, Portugal.ORCID 0000-0002-2342-3765
Ana M GilDepartment of Chemistry, University of Aveiro, CICECO-Aveiro Institute of Materials (CICECO/UA), 3810-193 Aveiro, Portugal.ORCID 0000-0003-3766-4364
University of Aveiro · PTUniversity of Chemistry and Technology, Prague · CZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This paper describes, for the first time to our knowledge, a lipidome and exometabolome characterization of osteogenic differentiation for human adipose tissue stem cells (hAMSCs) using nuclear magnetic resonance (NMR) spectroscopy. The holistic nature of NMR enabled the time-course evolution of cholesterol, mono- and polyunsaturated fatty acids (including ω-6 and ω-3 fatty acids), several phospholipids (phosphatidylcholine, phosphatidylethanolamine, sphingomyelins, and plasmalogens), and mono- and triglycerides to be followed. Lipid changes occurred almost exclusively between days 1 and 7, followed by a tendency for lipidome stabilization after day 7. On average, phospholipids and longer and more unsaturated fatty acids increased up to day 7, probably related to plasma membrane fluidity. Articulation of lipidome changes with previously reported polar endometabolome profiling and with exometabolome changes reported here in the same cells, enabled important correlations to be established during hAMSC osteogenic differentiation. Our results supported hypotheses related to the dynamics of membrane remodelling, anti-oxidative mechanisms, protein synthesis, and energy metabolism. Importantly, the observation of specific up-taken or excreted metabolites paves the way for the identification of potential osteoinductive metabolites useful for optimized osteogenic protocols.

Indexed as

Mesenchymal Stem CellsOsteogenesisCell DifferentiationFatty Acids, UnsaturatedHumansLipidomicsPhospholipidsFatty Acids, UnsaturatedPhospholipidsendometabolomeexometabolomelipidomicsmesenchymal stem cellsmetabolomicsNMR spectroscopyosteogenesisosteogenic differentiation

Identifiers

PMID35455937
PMCPMC9024772
OpenAlexW4224257209

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.