Evidence map›Paper›PMID 35456479›Full record

ArticleGenes2022

hMSH5 Regulates NHEJ and Averts Excessive Nucleotide Alterations at Repair Joints.

Aneesa T Al-Soodani, Xiling Wu, Nicole C Kelp, Alexander J Brown, Steven A Roberts, Chengtao Her

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 59% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Aneesa T Al-SoodaniSchool of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA.
Xiling WuSchool of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA.
Nicole C KelpSchool of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA.
Alexander J BrownSchool of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA.
Steven A RobertsSchool of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA.
Chengtao HerSchool of Molecular Biosciences, Washington State University, Pullman, WA 99164, USA.
Washington State University · US

Funding

TRAINING IN BIOTECHNOLOGY: EMPHASIS ON PROTEIN CHEMISTRYT32GM008336 · NIGMS · WASHINGTON STATE UNIVERSITY · PI CALL, DOUGLAS R. · 1989 to 2023
$8.9M
Mechanisms of genome instability induced by APOBEC Cytidine Deaminases & its impacts during cancer development - Diversity SupplementR01CA218112 · NCI · WASHINGTON STATE UNIVERSITY · PI ROBERTS, STEVEN A · 2017 to 2021
$2.2M
NCI NIH HHS R01 CA218112NIGMS NIH HHS T32 GM008336
6 · The paper itself

Abstract

Inappropriate repair of DNA double-strand breaks (DSBs) leads to genomic instability, cell death, or malignant transformation. Cells minimize these detrimental effects by selectively activating suitable DSB repair pathways in accordance with their underlying cellular context. Here, we report that hMSH5 down-regulates NHEJ and restricts the extent of DSB end processing before rejoining, thereby reducing "excessive" deletions and insertions at repair joints. RNAi-mediated knockdown of hMSH5 led to large nucleotide deletions and longer insertions at the repair joints, while at the same time reducing the average length of microhomology (MH) at repair joints. Conversely, hMSH5 overexpression reduced end-joining activity and increased RPA foci formation (i.e., more stable ssDNA at DSB ends). Furthermore, silencing of hMSH5 delayed 53BP1 chromatin spreading, leading to increased end resection at DSB ends.

Indexed as

DNA End-Joining RepairNucleotidesChromatinDNA Breaks, Double-StrandedDNA, Single-StrandedChromatinDNA, Single-StrandedNucleotides53BP1DSB repairend resectiongenome instabilityMSH5NHEJ

Identifiers

PMID35456479
PMCPMC9026759
OpenAlexW4223436237

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.