Evidence map›Paper›PMID 35497490›Full record

ArticleCell reports methods2022

Multimodal imaging of synaptic vesicles with a single probe.

Seong J An, Massimiliano Stagi, Travis J Gould, Yumei Wu, Michael Mlodzianoski, Felix Rivera-Molina, Derek Toomre, Stephen M Strittmatter, Pietro De Camilli, Joerg Bewersdorf and 1 more

Abstract read
In one paragraph

Article in Cell reports methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Phospholipase ACell reports methods · 2022
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Seong J AnDepartment of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, USA.
Massimiliano StagiProgram in Cellular Neuroscience, Neurodegeneration & Repair, Yale University School of Medicine, New Haven, CT 06510, USA.
Travis J GouldDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA.
Yumei WuProgram in Cellular Neuroscience, Neurodegeneration & Repair, Yale University School of Medicine, New Haven, CT 06510, USA.
Michael MlodzianoskiDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA.
Felix Rivera-MolinaDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA.
Derek ToomreDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA.
Stephen M StrittmatterProgram in Cellular Neuroscience, Neurodegeneration & Repair, Yale University School of Medicine, New Haven, CT 06510, USA.
Pietro De CamilliProgram in Cellular Neuroscience, Neurodegeneration & Repair, Yale University School of Medicine, New Haven, CT 06510, USA.
Joerg BewersdorfDepartment of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA.
David ZenisekDepartment of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510, USA.

Funding

Yale Diabetes Research CenterP30DK045735 · NIDDK · YALE UNIVERSITY · PI GERALD I SHULMAN · 1993 to 2026
$44.0M
Prion Protein in Alzheimer's Disease PathophysiologyR01AG034924 · NIA · YALE UNIVERSITY · PI STEPHEN M STRITTMATTER · 2010 to 2026
$9.6M
Genome-Wide Discovery and Translational Research for Neural RepairR35NS097283 · NINDS · YALE UNIVERSITY · PI STRITTMATTER, STEPHEN M · 2017 to 2024
$8.1M
Yale Core Grant for Vision ResearchP30EY026878 · NEI · YALE UNIVERSITY · PI Jonathan B Demb · 2016 to 2026
$7.1M
Yale Core Grant For Vision ResearchP30EY000785 · NEI · YALE UNIVERSITY · PI CRAIR, MICHAEL C. · 1985 to 2010
$5.8M
MOLECULAR MECHANISMS IN SYNAPTIC VESICLE RECYCLINGR01NS036251 · NINDS · YALE UNIVERSITY · PI DE CAMILLI, PIETRO · 1997 to 2021
$4.7M
Investigating the Role of Ribeye in Retinal Ribbon FunctionR01EY021195 · NEI · YALE UNIVERSITY · PI ZENISEK, DAVID PAUL · 2011 to 2019
$3.3M
Mechanisms of the calcium-triggered neurotransmitter release machinery in hair cellsR01DC019057 · NIDCD · YALE UNIVERSITY · PI KARATEKIN, ERDEM, ZENISEK, DAVID PAUL · 2020 to 2024
$2.7M
Molecular Mechanisms in Synaptic Vesicle RecyclingR37NS036251 · NINDS · YALE UNIVERSITY · PI DE CAMILLI, PIETRO · 2010 to 2016
$2.7M
Synaptic transmission at retinal ribbon synapsesR01EY032396 · NEI · YALE UNIVERSITY · PI THORESON, WALLACE B, ZENISEK, DAVID PAUL · 2021 to 2024
$1.9M
Progranulin interaction with Sortilin in FrontoTemporal DementiaR01NS074319 · NINDS · YALE UNIVERSITY · PI STRITTMATTER, STEPHEN M · 2011 to 2015
$1.8M
Dynamic and super-resolution imaging of endogenous proteins in Drosophila tissuesRC1GM091791 · NIGMS · YALE UNIVERSITY · PI COOLEY, LYNN · 2009 to 2010
$947k
NEI NIH HHS P30 EY000785NEI NIH HHS P30 EY026878NEI NIH HHS R01 EY021195NEI NIH HHS R01 EY032396NIA NIH HHS R01 AG034924NIDCD NIH HHS R01 DC019057NIDDK NIH HHS P30 DK045735NIGMS NIH HHS RC1 GM091791NINDS NIH HHS R01 NS036251NINDS NIH HHS R01 NS074319NINDS NIH HHS R35 NS097283NINDS NIH HHS R37 NS036251Wellcome Trust 203285/Z/16/Z
6 · The paper itself

Abstract

A complete understanding of synaptic-vesicle recycling requires the use of multiple microscopy methods to obtain complementary information. However, many currently available probes are limited to a specific microscopy modality, which necessitates the use of multiple probes and labeling paradigms. Given the complexity of vesicle populations and recycling pathways, having new single-vesicle probes that could be used for multiple microscopy techniques would complement existing sets of tools for studying vesicle function. Here, we present a probe based on the membrane-binding C2 domain of cytosolic phospholipase A

Indexed as

Multimodal ImagingSynaptic VesiclesC2 domainelectron microscopyPALMSTEDsuper-resolutionsynapsesynaptic vesiclesTIRF

Identifiers

PMID35497490
PMCPMC9046237

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.