Evidence map›Paper›PMID 35526246›Full record

ArticleMolecular biology reports2022

Expression of the GFP-mammalian pleckstrin homology (PH) domain of the phospholipase C δ1 in Saccharomyces cerevisiae BY4741.

Francine Perrine-Walker, Jennifer Payne

Abstract read
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In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. PI(4,5)PBio-protocol · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 2 countries.

Francine Perrine-WalkerDepartment of Biochemistry and Genetics, La Trobe Institute For Molecular Science, La Trobe University, 3086, Bundoora, VIC, Australia. marie.perrine-walker@sydney.edu.au.ORCID http://orcid.org/0000-0002-9895-232X
Jennifer PayneDepartment of Biochemistry and Genetics, La Trobe Institute For Molecular Science, La Trobe University, 3086, Bundoora, VIC, Australia.
La Trobe University · AUThe University of Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPleckstrin homology (PH) domains are common modules of ∼120 amino acids found in proteins involved in signalling, cytoskeletal organization, membrane transport, and modification of phospholipids. Previous live cell studies have involved the use of the green-fluorescent protein (GFP) labelling of PH-domain of phospholipase C δ1 (PLC δ1) to study the interactions of molecules at the membrane interface. METHODS AND

resultsFor this study, the aim was to construct and express the GFP-PH domain of PLC δ1 in the Saccharomyces cerevisiae BY4741. The transformants expressing GFP-PH domain of PLC δ1 displayed localised fluorescence to the cell periphery (plasma membrane) while the negative control expressed GFP within the cytoplasm only. No GFP was observed in the non-transformed yeast cells.

conclusionsThus, this technique could be useful in future molecular interactions studies targeted specifically at the yeast cell membrane interface in live yeast cells.

Indexed as

Pleckstrin Homology DomainsSaccharomyces cerevisiaeAnimalsBlood ProteinsCell MembraneGreen Fluorescent ProteinsMammalsPhospholipase C deltaPhosphoproteinsType C PhospholipasesBlood ProteinsGreen Fluorescent ProteinsPhospholipase C deltaPhosphoproteinsplatelet protein P47Type C PhospholipasesConfocal microscopyGreen fluorescent proteinYeast

Identifiers

PMID35526246
OpenAlexW4229332881

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.