Evidence mapPaperPMID 35561211Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2022

Structural basis of peptidomimetic agonism revealed by small- molecule GLP-1R agonists Boc5 and WB4-24.

Zhaotong Cong, Qingtong Zhou, Yang Li, Li-Nan Chen, Zi-Chen Zhang, Anyi Liang, Qing Liu, Xiaoyan Wu, Antao Dai, Tian Xia and 4 more

Open access · greenAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
2.8field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 33 citations in OpenAlex.

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  13. Structural analysis of the dual agonism at GLP-1R and GCGR.Proceedings of the National Academy of Sciences of the United States of America · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 1 country.

Zhaotong CongDepartment of Pharmacology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Qingtong ZhouDepartment of Pharmacology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.ORCID 0000-0001-8124-3079
Yang LiDepartment of Pharmacology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.
Li-Nan ChenDepartment of Biophysics, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.
Zi-Chen ZhangSchool of Pharmacy, Fudan University, Shanghai 201203, China.
Anyi LiangSchool of Artificial Intelligence and Automation, Huazhong University of Science and Technology, Wuhan 430074, China.
Qing LiuThe National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.ORCID 0000-0003-1825-6760
Xiaoyan WuThe National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Antao DaiThe National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Tian XiaSchool of Artificial Intelligence and Automation, Huazhong University of Science and Technology, Wuhan 430074, China.
Wei WuSchool of Pharmacy, Fudan University, Shanghai 201203, China.
Yan ZhangDepartment of Biophysics, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310058, China.ORCID 0000-0003-2189-0244
Dehua YangThe National Center for Drug Screening, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.ORCID 0000-0003-3028-3243
Ming-Wei WangDepartment of Pharmacology, School of Basic Medical Sciences, Fudan University, Shanghai 200032, China.ORCID 0000-0001-6550-9017
Fudan University · CNShanghai Institute of Materia Medica · CNChinese Academy of Sciences · CNHuazhong University of Science and Technology · CNSir Run Run Shaw Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glucagon-like peptide-1 receptor (GLP-1R) agonists are effective in treating type 2 diabetes and obesity with proven cardiovascular benefits. However, most of these agonists are peptides and require subcutaneous injection except for orally available semaglutide. Boc5 was identified as the first orthosteric nonpeptidic agonist of GLP-1R that mimics a broad spectrum of bioactivities of GLP-1 in vitro and in vivo. Here, we report the cryoelectron microscopy structures of Boc5 and its analog WB4-24 in complex with the human GLP-1R and Gs protein. Bound to the extracellular domain, extracellular loop 2, and transmembrane (TM) helices 1, 2, 3, and 7, one arm of both compounds was inserted deeply into the bottom of the orthosteric binding pocket that is usually accessible by peptidic agonists, thereby partially overlapping with the residues A8 to D15 in GLP-1. The other three arms, meanwhile, extended to the TM1-TM7, TM1-TM2, and TM2-TM3 clefts, showing an interaction feature substantially similar to the previously known small-molecule agonist LY3502970. Such a unique binding mode creates a distinct conformation that confers both peptidomimetic agonism and biased signaling induced by nonpeptidic modulators at GLP-1R. Further, the conformational difference between Boc5 and WB4-24, two closed related compounds, provides a structural framework for fine-tuning of pharmacological efficacy in the development of future small-molecule therapeutics targeting GLP-1R.

Indexed as

CyclobutanesGlucagon-Like Peptide-1 ReceptorGlucagon-Like Peptide-1 Receptor AgonistsPeptidomimeticsCryoelectron MicroscopyHumansProtein DomainsBoc5 compoundCyclobutanesGlucagon-Like Peptide-1 ReceptorGlucagon-Like Peptide-1 Receptor AgonistsPeptidomimeticsWB4-24cryoelectron microscopyglucagon-like peptide-1 receptorpeptidomimetic agonismtype 2 diabetes mellitus

Identifiers

PMID35561211
PMCPMC9171782
OpenAlexW4280502247

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.