Determinants of RPA megafoci localization to the nuclear periphery in response to replication stress.

Abstract read

In one paragraph

Article in G3 (Bethesda, Md.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

A systematic quantitative approach comprehensively defines domain-specific functional pathways linked to Schizosaccharomyces pombe heterochromatin regulation.Nucleic acids research · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Seong Min KimMolecular & Computational Biology, University of Southern California, Los Angeles, CA 90007, USA.ORCID 0000-0001-8328-8211

Susan L ForsburgMolecular & Computational Biology, University of Southern California, Los Angeles, CA 90007, USA.ORCID 0000-0002-4895-8598

Funding

Mechanisms linking replication stress to genome instability in fission yeastR35GM118109 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI FORSBURG, SUSAN L · 2016 to 2025

$7.1M

NIGMS NIH HHS R35 GM118109

6 · The paper itself

Abstract

Upon replication stress, ssDNA, coated by the ssDNA-binding protein RPA, accumulates and generates a signal to activate the replication stress response. Severe replication stress induced by the loss of minichromosome maintenance helicase subunit Mcm4 in the temperature-sensitive Schizosaccharomyces pombe degron mutant (mcm4-dg) results in the formation of a large RPA focus that is translocated to the nuclear periphery. We show that resection and repair processes and chromatin remodeler Swr1/Ino80 are involved in the large RPA foci formation and its relocalization to nuclear periphery. This concentrated accumulation of RPA increases the recruitment of Cds1 to chromatin and results in an aberrant cell cycle that lacks MBF-mediated G1/S accumulation of Tos4. These findings reveal a distinct replication stress response mediated by localized accumulation of RPA that allows the evasion of cell cycle arrest.

Indexed as

SchizosaccharomycesSchizosaccharomyces pombe ProteinsChromatinDNA DamageDNA HelicasesDNA RepairDNA ReplicationMinichromosome Maintenance Complex Component 4Replication Protein ATranscription FactorsChromatinDNA HelicasesIno80 protein, S pombemcm4 protein, S pombeMinichromosome Maintenance Complex Component 4Replication Protein ASchizosaccharomyces pombe ProteinsTranscription FactorscheckpointDNA repairMCM4Replication stressRPA

Identifiers

PMID35567482

DOI10.1093/g3journal/jkac116

PMCPMC9258583

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.